Fig. 3.

Incorporation of [1-¹⁴C]dodecanoic acid or [1-¹⁴C]tetradecanoic acid into the phospholipid (Panel A) or lipid A (Panel B) acyl chains of strain K19 (fadE62) carrying either the vector plasmid pBAD24 or the AasS encoding plasmid pYFJ85 assayed by reverse phase chromatography. The phospholipid acyl chains were analyzed as their methyl esters whereas the lipid A acyl chains were analyzed by reverse phase chromatography as free fatty acids. Note than the right hand two lanes of panel B were exposed three times as long as the two left hand lanes (two exposures of the same plate were done). Arabinose was present at 0.1%. C12, C14 and C16 denote dodecanoic, tetradecanoic and hexadecanoic acids, respectively, whereas 3-OH C14 denotes 3-hydroxytetradecanoic acid. The fatty acids were identified using authentic standards as described previously (19).