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. 1980 Sep;35(3):854–864. doi: 10.1128/jvi.35.3.854-864.1980

Two-dimensional analysis of proteins sedimenting with simian virus 40 chromosomes.

B I Milavetz, L D Spotila, R Thomas, J A Huberman
PMCID: PMC288879  PMID: 6252343

Abstract

The nonhistone proteins sedimenting in low-salt glycerol gradients with simian virus 40 chromosomes were analyzed by two-dimensional gel electrophoresis, utilizing nonequilibrium pH gradients as the first dimension and sodium dodecyl sulfate-gel electrophoresis as the second dimension. By densitometric quantitation of the radiolabeled proteins present in each fraction of the gradients, it was possible to identify sedimenting with all or a fraction of the simian virus 40 chromosomes. VP-1 sedimented with simian virus 40 chromosomes; additional evidence for its binding to chromosomes was obtained by immunochemical techniques. Four proteins (Mr 25,000, pI 6.0; Mr 32,000, pI 7.2; Mr 35,000, pI 8.5; and Mr 80,000, pI 7.2) sedimented with specific subsets of chromosomes.

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Selected References

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