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. 1980 Sep;35(3):902–917. doi: 10.1128/jvi.35.3.902-917.1980

Preparation and characterization of specific antisera to individual glycoprotein antigens comprising the major glycoprotein region of herpes simplex virus type 1.

R Eberle, R J Courtney
PMCID: PMC288884  PMID: 6252346

Abstract

The major glycoprotein complex (VP123) of herpes simplex virus type 1 resolved by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis was purified and further fractionated into two major and two minor components by chromatography of the isolated VP123 region on SDS-hydroxylapatite columns. The two major components (gC and gA/gB) were purified free of other polypeptides and used to prepare specific antisera to these glycoproteins. Radioimmune precipitation demonstrated that these antisera were specific for the antigens used in their production. These two antisera as well as an anti-VP123 serum were further characterized by immunoprecipitation, neutralization, and membrane immunofluorescence techniques. Results indicate that both of the major glycoprotein antigens are expressed on the surface of virions as well as on the surface of infected cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bone D. R., Courtney R. J. A temperature-sensitive mutant of herpes simplex virus type 1 defective in the synthesis of the major capsid polypeptide. J Gen Virol. 1974 Jul;24(1):17–27. doi: 10.1099/0022-1317-24-1-17. [DOI] [PubMed] [Google Scholar]
  2. Cohen G. H., Katze M., Hydrean-Stern C., Eisenberg R. J. Type-common CP-1 antigen of herpes simplex virus is associated with a 59,000-molecular-weight envelope glycoprotein. J Virol. 1978 Jul;27(1):172–181. doi: 10.1128/jvi.27.1.172-181.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Courtney R. J., Benyesh-Melnick M. Isolation and characterization of a large molecular-weight polypeptide of herpes simplex virus type 1. Virology. 1974 Dec;62(2):539–551. doi: 10.1016/0042-6822(74)90414-0. [DOI] [PubMed] [Google Scholar]
  4. Courtney R. J. Immunologic and biochemical characterization of individual polypeptides induced by herpes simplex virus types 1 and 2. Adv Pathobiol. 1976;(5):87–103. [PubMed] [Google Scholar]
  5. Courtney R. J., Powell K. L. Immunological and biochemical characterization of polypeptides induced by herpes simplex virus types 1 and 2. IARC Sci Publ. 1975;(11 Pt 1):63–73. [PubMed] [Google Scholar]
  6. Dreesman G. R., Matson D. O., Courtney R. J., Adam E., Melnick J. L. Detection of herpesvirus type-specific antibody by a micro solid phase radioimmunometric assay. Intervirology. 1979;12(2):115–119. doi: 10.1159/000149077. [DOI] [PubMed] [Google Scholar]
  7. Ejercito P. M., Kieff E. D., Roizman B. Characterization of herpes simplex virus strains differing in their effects on social behaviour of infected cells. J Gen Virol. 1968 May;2(3):357–364. doi: 10.1099/0022-1317-2-3-357. [DOI] [PubMed] [Google Scholar]
  8. Gibson W., Roizman B. Proteins specified by herpes simplex virus. Staining and radiolabeling properties of B capsid and virion proteins in polyacrylamide gels. J Virol. 1974 Jan;13(1):155–165. doi: 10.1128/jvi.13.1.155-165.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Glorioso J. C., Smith J. W. Immune interactions with cells infected with herpes simplex virus: antibodies to radioiodinated surface antigens. J Immunol. 1977 Jan;118(1):114–121. [PubMed] [Google Scholar]
  10. Heine J. W., Spear P. G., Roizman B. Proteins specified by herpes simplex virus. VI. Viral proteins in the plasma membrane. J Virol. 1972 Mar;9(3):431–439. doi: 10.1128/jvi.9.3.431-439.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Honess R. W., Powell K. L., Robinson D. J., Sim C., Watson D. H. Type specific and type common antigens in cells infected with herpes simplex virus type 1 and on the surfaces of naked and enveloped particles of the virus. J Gen Virol. 1974 Feb;22(2):159–169. doi: 10.1099/0022-1317-22-2-159. [DOI] [PubMed] [Google Scholar]
  12. Jeansson S. Preparation of type specific herpes simplex antisera by an immunosorbent method;. Acta Pathol Microbiol Scand B. 1975 Feb;83(1):48–54. doi: 10.1111/j.1699-0463.1975.tb00069.x. [DOI] [PubMed] [Google Scholar]
  13. Moss B., Rosenblum E. N. Hydroxylapatite chromatography of protein-sodium dodecyl sulfate complexes. A new method for the separation of polypeptide subunits. J Biol Chem. 1972 Aug 25;247(16):5194–5198. [PubMed] [Google Scholar]
  14. Nahmias A. J., Josey W. E., Naib Z. M., Luce C. F., Guest B. A. Antibodies to Herpesvirus hominis types 1 and 2 in humans. II. Women with cervical cancer. Am J Epidemiol. 1970 Jun;91(6):547–552. doi: 10.1093/oxfordjournals.aje.a121166. [DOI] [PubMed] [Google Scholar]
  15. Norrild B., Ludwig H., Rott R. Identification of a common antigen of herpes simplex virus bovine herpes mammillitis virus, and B virus. J Virol. 1978 Jun;26(3):712–717. doi: 10.1128/jvi.26.3.712-717.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Norrild B., Vestergaard B. F. Polyacrylamide gel electrophoretic analysis of herpes simplex virus type 1 immunoprecipitates obtained by quantitative immunoelectrophoresis in antibody-containing agarose gel. J Virol. 1977 Apr;22(1):113–117. doi: 10.1128/jvi.22.1.113-117.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Olshevsky U., Becker Y. Surface glycopeptides in the envelope of herpes simplex virions. Virology. 1972 Oct;50(1):277–279. doi: 10.1016/0042-6822(72)90371-6. [DOI] [PubMed] [Google Scholar]
  18. PLUMMER G. SEROLOGICAL COMPARISON OF THE HERPES VIRUSES. Br J Exp Pathol. 1964 Apr;45:135–141. [PMC free article] [PubMed] [Google Scholar]
  19. Pauli G., Ludwig H. Immunoprecipitation of herpes simplex virus type 1 antigens with different antisera and human cerebrospinal fluids. Arch Virol. 1977;53(1-2):139–155. doi: 10.1007/BF01314855. [DOI] [PubMed] [Google Scholar]
  20. Powell K. L., Buchan A., Sim C., Watson D. H. Type-specific protein in herpes simplex virus envelope reacts with neutralising antibody. Nature. 1974 May 24;249(455):360–361. doi: 10.1038/249360a0. [DOI] [PubMed] [Google Scholar]
  21. Powell K. L., Courtney R. J. Polypeptide synthesized in herpes simplex virus type 2-infected HEp-2 cells. Virology. 1975 Jul;66(1):217–228. doi: 10.1016/0042-6822(75)90192-0. [DOI] [PubMed] [Google Scholar]
  22. Rawls W. E., Iwamoto K., Adam E., Melnick J. L. Measurement of antibodies to herpesvirus types 1 and 2 in human sera. J Immunol. 1970 Mar;104(3):599–606. [PubMed] [Google Scholar]
  23. Reynolds J. A., Tanford C. Binding of dodecyl sulfate to proteins at high binding ratios. Possible implications for the state of proteins in biological membranes. Proc Natl Acad Sci U S A. 1970 Jul;66(3):1002–1007. doi: 10.1073/pnas.66.3.1002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sarmiento M., Haffey M., Spear P. G. Membrane proteins specified by herpes simplex viruses. III. Role of glycoprotein VP7(B2) in virion infectivity. J Virol. 1979 Mar;29(3):1149–1158. doi: 10.1128/jvi.29.3.1149-1158.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Seth P., Prakash S. S., Ghosh D. Antibodies to Herpes simplex virus types 1 and 2 in patients with squamous-cell carcinoma of uterine cervix in India. Int J Cancer. 1978 Dec;22(6):708–714. doi: 10.1002/ijc.2910220612. [DOI] [PubMed] [Google Scholar]
  26. Sim C., Watson D. H. The role of type specific and cross reacting structural antigens in the neutralization of herpes simplex virus types 1 and 2. J Gen Virol. 1973 May;19(2):217–233. doi: 10.1099/0022-1317-19-2-217. [DOI] [PubMed] [Google Scholar]
  27. Skinner G. R., Thouless M. E., Trueman S., Edwards J., Gibbs A. J. Serological relatedness of herpes simplex viruses. Type-specificity of antibody response. Immunology. 1976 Sep;31(3):481–494. [PMC free article] [PubMed] [Google Scholar]
  28. Spear P. G. Glycoproteins specified by herpes simplex virus type 1: their synthesis, processing and antigenic relatedness to HSV -2 glycoproteins. IARC Sci Publ. 1975;(11 Pt 1):49–61. [PubMed] [Google Scholar]
  29. Spear P. G. Membrane proteins specified by herpes simplex viruses. I. Identification of four glycoprotein precursors and their products in type 1-infected cells. J Virol. 1976 Mar;17(3):991–1008. doi: 10.1128/jvi.17.3.991-1008.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Van Eldik L. J., Paulson J. C., Green R. W., Smith R. E. The influence of carbohydrate on the antigenicity of the envelope glycoprotein of avian myeloblastosis virus and B77 avian sarcoma virus. Virology. 1978 May 1;86(1):193–204. doi: 10.1016/0042-6822(78)90020-x. [DOI] [PubMed] [Google Scholar]
  31. Vestergaard B. F., Norrild B. Crossed immunoelectrophoresis of a herpes simplex virus type 1-specific antigen: immunological and biochemical characterization. J Infect Dis. 1978 Nov;138(5):639–643. doi: 10.1093/infdis/138.5.639. [DOI] [PubMed] [Google Scholar]

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