Abstract
Bacteriophage Psp231a infects Pseudomonas phaseolicola, strain HB10Y, which is the host cell for the enveloped bacteriophage phi 6. This paper describes the biophysical characteristics of Psp231a and the physical properties of its nucleic acid. In electron micrographs the virion appears as an icosahedral structure, approximately 55 nm in diameter, with a short tail. The virion density is 1.48 g/cm3 in CsCl, and the sedimentation coefficient is approximately 407S. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of 12 polypeptides ranging in molecular weight from 5,000 to 117,000. The nucleic acid of Psp231a is linear, double-stranded DNA of molecular weight 28 X 10(6). Its density in CsCl is 1.716 g/cm3, and its sedimentation coefficient in 3 M CsCl is 20.0S, corresponding to an S020,W of 34S.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ackermann H. W., Audurier A., Berthiaume L., Jones L. A., Mayo J. A., Vidaver A. K. Guidelines for bacteriophage characterization. Adv Virus Res. 1978;23:1–24. doi: 10.1016/s0065-3527(08)60096-2. [DOI] [PubMed] [Google Scholar]
- Fisher H. W., Williams R. C. Electron microscopic visualization of nucleic acids and of their complexes with proteins. Annu Rev Biochem. 1979;48:649–679. doi: 10.1146/annurev.bi.48.070179.003245. [DOI] [PubMed] [Google Scholar]
- Hayward G. S., Smith M. G. The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis. J Mol Biol. 1972 Feb 14;63(3):383–395. doi: 10.1016/0022-2836(72)90435-4. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Lang D., Mitani M. Simplified quantitative electron microscopy of biopolymers. Biopolymers. 1970;9(3):373–379. doi: 10.1002/bip.1970.360090310. [DOI] [PubMed] [Google Scholar]
- SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
- Snipes W., Keller G., Woog J., Vickroy T., Deering R., Keith A. Inactivation of lipid-containing viruses by hydrophobic photosensitizers and near-ultraviolet radiation. Photochem Photobiol. 1979 Apr;29(4):785–790. doi: 10.1111/j.1751-1097.1979.tb07767.x. [DOI] [PubMed] [Google Scholar]
- Snipes W., Person S., Keller G., Taylor W., Keith A. Inactivation of lipid-containing viruses by long-chain alcohols. Antimicrob Agents Chemother. 1977 Jan;11(1):98–104. doi: 10.1128/aac.11.1.98. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vidaver A. K., Koski R. K., Van Etten J. L. Bacteriophage phi6: a Lipid-Containing Virus of Pseudomonas phaseolicola. J Virol. 1973 May;11(5):799–805. doi: 10.1128/jvi.11.5.799-805.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wanda P., Cupp J., Snipes W., Deith A., Rucinsky T., Polish L., Sands J. Inactivation of the enveloped bacteriophage phi6 by butylated hydroxytoluene and butylated hydroxyanisole. Antimicrob Agents Chemother. 1976 Jul;10(1):96–101. doi: 10.1128/aac.10.1.96. [DOI] [PMC free article] [PubMed] [Google Scholar]