Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1986 Nov;60(2):787–792. doi: 10.1128/jvi.60.2.787-792.1986

Cloning the complete rIIB gene of bacteriophage T4 and some observations concerning its middle promoters.

S Shinedling, L T Walker, L Gold
PMCID: PMC288959  PMID: 3022011

Abstract

We cloned the intact T4 rIIB gene by joining plasmids carrying gene fragments. rIIB was expressed at a low level under control of the lac promoter, and the clone complemented rIIB mutants. We suspect that earlier attempts to clone the intact gene were unsuccessful because of transcription from T4 middle-mode promoters. These promoters are silent early in infection but are recognized when resident on a plasmid in an uninfected cell.

Full text

PDF
787

Images in this article

790Image
on p.790
791Image
on p.791

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Eisenbeis S. J., Nasoff M. S., Noble S. A., Bracco L. P., Dodds D. R., Caruthers M. H. Altered Cro repressors from engineered mutagenesis of a synthetic cro gene. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1084–1088. doi: 10.1073/pnas.82.4.1084. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Guarente L., Lauer G., Roberts T. M., Ptashne M. Improved methods for maximizing expression of a cloned gene: a bacterium that synthesizes rabbit beta-globin. Cell. 1980 Jun;20(2):543–553. doi: 10.1016/0092-8674(80)90640-6. [DOI] [PubMed] [Google Scholar]
  3. Manoil C., Sinha N., Alberts B. Intracellular DNA-protein complexes from bacteriophage T4-infected cells isolated by a rapid two-step procedure. Characterization and identification of the protein components. J Biol Chem. 1977 Apr 25;252(8):2734–2741. [PubMed] [Google Scholar]
  4. Mattson T., Richardson J., Goodin D. Mutant of bacteriophage T4D affecting expression of many early genes. Nature. 1974 Jul 5;250(461):48–50. doi: 10.1038/250048a0. [DOI] [PubMed] [Google Scholar]
  5. Parker R. C., Watson R. M., Vinograd J. Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis. Proc Natl Acad Sci U S A. 1977 Mar;74(3):851–855. doi: 10.1073/pnas.74.3.851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Parma D. H., Dill M., Slocum M. K. Realignment of the genetic map of the terminus of the rIIB cistron of bacteriophage T4. Genetics. 1979 Jul;92(3):711–720. doi: 10.1093/genetics/92.3.711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Pribnow D., Sigurdson D. C., Gold L., Singer B. S., Napoli C., Brosius J., Dull T. J., Noller H. F. rII cistrons of bacteriophage T4. DNA sequence around the intercistronic divide and positions of genetic landmarks. J Mol Biol. 1981 Jul 5;149(3):337–376. doi: 10.1016/0022-2836(81)90477-0. [DOI] [PubMed] [Google Scholar]
  8. Sadler J. R., Tecklenburg M., Betz J. L. Plasmids containing many tandem copies of a synthetic lactose operator. Gene. 1980 Feb;8(3):279–300. doi: 10.1016/0378-1119(80)90005-0. [DOI] [PubMed] [Google Scholar]
  9. Sanzey B. Modulation of gene expression by drugs affecting deoxyribonucleic acid gyrase. J Bacteriol. 1979 Apr;138(1):40–47. doi: 10.1128/jb.138.1.40-47.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Selzer G., Belin D., Bolle A., Van Houwe G., Mattson T., Epstein R. In vivo expression of the rII region of bacteriophage T4 present in chimeric plasmids. Mol Gen Genet. 1981;183(3):505–513. doi: 10.1007/BF00268772. [DOI] [PubMed] [Google Scholar]
  11. Selzer G., Bolle A., Krisch B., Epstein R. Construction and properties of recombinant plasmids containing the rII genes of bacteriophage T4. Mol Gen Genet. 1978 Feb 27;159(3):301–309. doi: 10.1007/BF00268267. [DOI] [PubMed] [Google Scholar]
  12. Shuman H., Schwartz M. The effect of nalidixic acid on the expression of some genes in Escherichia coli K-12. Biochem Biophys Res Commun. 1975 May 5;64(1):204–209. doi: 10.1016/0006-291x(75)90239-9. [DOI] [PubMed] [Google Scholar]
  13. Singer B. S., Gold L. A mutation that confers temperature sensitivity on the translation of rIIB in bacteriophage T4. J Mol Biol. 1976 May 25;103(3):627–646. doi: 10.1016/0022-2836(76)90221-7. [DOI] [PubMed] [Google Scholar]
  14. Smith C. L., Kubo M., Imamoto F. Promoter-specific inhibition of transcription by antibiotics which act on DNA gyrase. Nature. 1978 Oct 5;275(5679):420–423. doi: 10.1038/275420a0. [DOI] [PubMed] [Google Scholar]
  15. Yang H. L., Heller K., Gellert M., Zubay G. Differential sensitivity of gene expression in vitro to inhibitors of DNA gyrase. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3304–3308. doi: 10.1073/pnas.76.7.3304. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES