Table 3.
Yeast TIM |
kcatb S−1 |
Kmb mM |
kcat/Km M−1 s−1 |
Ki for PGA mM |
---|---|---|---|---|
Wildtype | 7300 ± 400 | 1.1 ± 0.2 | 6.6 × 106c | 0.019 ± 0.004d |
K12G | 0.6 ± 0.2 | 50 ± 20 | 12 ± 0.4 e | 1.1 ± 0.2f |
Effect g | 1.2 × 104 | 50 | 5.5 × 105 | 60 |
ΔΔG or ΔΔG‡ kcal/mol |
5.6 | 2.3 | 7.8 | 2.4 |
At pH 7.5 (30 mM TEA), 25 °C and I = 0.10 (NaCl). Quoted errors are standard errors obtained from least squares analysis.
Determined from the fit of initial velocity data to the Michaelis-Menten equation.
Calculated as the ratio of the values of kcat and Km
Determined by global nonlinear least squares analysis of initial velocity data in the presence of zero, 21 and 130 µM PGA.
Determined as the slope of the plot of vi/[E] against [GAP] for [GAP] ≤ 3 mM (Figure 4, inset).
The effect of the K12G mutation on the kinetic parameter.