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DNA damage in sperm from three donors, exposed to 10 or 25 μM B[a]P (without human S9-mix). Data were assessed by one-way analysis of variance with a Dunnett's post-hoc test. The dose response is presented as mean % Tail DNA ± 1SE. Each treatment group was compared to the control group (1% DMSO); *P < 0.05. Experiments were repeated several times for each donor (unexposed controls, n = 10, 10 or 25 μM B[a]P, n = 4). Comet assay Protocol 1.
