Figure 6.

Differential effects of HDAC inhibitor treatment on H3- and H4- acetylation. CD34⁺ cells were cultured in the presence of G-CSF to induce neutrophil differentiation. Cells were treated overnight with TSA (5–25 nM), SB (100–500 μM) or VPA (100–500 μM) at days 3 and day 7. Lysates were made and western blot analysis was performed with antibodies against acetylated lysines, acetylated H3K9 and acetylated H4K16. As a control for equal loading an antibody against total H3 and an antibody against tubulin was used (n=2)