Figure 5.

CXCR4 blockade restores sensitivity of CD34⁺ CML progenitors to imatinib overcoming MSC protection: 5x10⁴ CD34⁺ CML cells were cultured alone or in the presence of MSC. After 48 h imatinib was added at different concentrations and the fraction of annexin-V-positive cells was quantified by FACS after a further 48 h. A representative dot plot for annexin-positive cells (A) and average histograms from three individual experiments (B) are shown.