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. Author manuscript; available in PMC: 2010 Jul 6.
Published in final edited form as: Biochemistry. 1994 May 24;33(20):6100–6109. doi: 10.1021/bi00186a009

Table 2.

Tyrosine Substitutions at Phenylalanine Positions: Effect on Ligand Binding

Mutant glutamate (mM) KDa
N36C 2.0 ± 0.2
N36C/F30Y 3.2 ± 0.3
N36C/F40Y 2.2 ± 0.4
N36C/F107Y 1.4 ± 0.1
N36C/F140Y 2.2 ± 0.6
N36C/F150Y 0.5 ± 0.3
N36C/F180Y 1.3 ± 0.8
a

Measured by monitoring intrinsic tryptophan fluorescence during glutamate titration at 25 °C in 10 mM Tris, pH 8.0 with HCl, 50 mM NaCl, 50 mM KCl, and 1 mM MgCl2. The concentration of the dimeric domain was 2.5 µM.