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. Author manuscript; available in PMC: 2011 Jul 6.
Published in final edited form as: Biochemistry. 2010 Jul 6;49(26):5570–5581. doi: 10.1021/bi100656j

FIGURE 4.

FIGURE 4

FANCA and FANCG, but not FANCC bind directly to μ-calpain. Yeast two-hybrid analysis of the interaction of FANCA, FANCC and FANCG to μ-calpain was examined. (A) LexA-FANCA or LexA-FANCC fusion proteins were co-expressed in yeast with either an empty vector (−) or the B42 construct containing μ-calpain; B42-FANCG fusion protein was coexpressed in yeast with an empty vector or a LexA-μ-calpain construct. Six randomly selected colonies from each of these yeast transformations were replica plated in a row to plates containing X-gal to test for activity for the reporter gene, β-galactosidase. Positive interaction between fusion proteins is seen by the presence of blue colonies, which indicates that the reporter gene has been activated. White colonies indicate no positive protein interactions. (B) Summary of the results of yeast two-hybrid analysis of the interaction of FANCA, FANCC and FANCG with μ-calpain: (+) good binding; (−) no binding.