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Incorporation of a single dGTP by Pfu-Pol into primer-templates containing varying bases in the template strand at +4, under single turnover conditions. Initially kobs was determined at various dGTP concentrations (data not shown) and used to generate secondary plots of kobs against [dGTP] for determination of kinetic parameters. A: diFBz+4 (●). B: T+4 (control) (■), FbzIm+4 (●). Kinetic parameters are summarized in table 3.
