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. Author manuscript; available in PMC: 2010 Jul 16.
Published in final edited form as: Biochemistry. 2008 Mar 26;47(16):4808–4816. doi: 10.1021/bi702494q

Figure 6.

Figure 6

Enzymatic activities of LeuRS wild type and N-terminal β-strand mutants. A. Leucylation activities were measured using enzyme and tRNALeu concentrations of 50 nM and 4 µM, respectively. B. Amino acid editing activities were determined using approximately 4 µM [3H]-Ile-tRNALeu and were initiated with 100 nM enzyme. Symbols are represented as follows: wild type (■), ΔS227E228 (▲), ΔS227 (▼), ΔE228 (◆) and no enzyme (X). Error bars are the result of reactions repeated in triplicate and are indicated for each point. C. CD spectra. The dark symbols represent the wild type LeuRS spectrum, while the light symbols overlap for the spectra of the three deletion mutants.