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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Traffic. 2009 Nov 19;11(2):185–197. doi: 10.1111/j.1600-0854.2009.01010.x

Figure 1.

Figure 1

Htz1 associates with Kap114, Kap123, and Nap1 in yeast cytosol. (A) Htz1-PrA and associated proteins were purified from cytosol prepared from a strain expressing Kap114-13myc, eluted with a MgCl2 step gradient, separated by SDS-PAGE and analyzed by Western blot (WB) using anti-Myc and anti-Nap1 antibodies. Htz1-PrA is recognized by IgG. (B) Nap1-PrA and associated proteins were purified from cytosol as above and probed with anti-Htz1 antibodies. (C) Htz1-PrA was purified from a strain expressing Kap123-13myc or (D) Kap120-13myc and analyzed as in (A). `Un' lane contains 0.02% of depleted post-bead starting cytosol. (E) Htz1-PrA was purified as in (A) and probed with anti-Chz1 and anti-Nap1 antibodies. W lane contains the last wash fraction. * indicates a proteolytic product of Htz1-PrA.