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. Author manuscript; available in PMC: 2010 Jul 26.
Published in final edited form as: Nature. 2005 Aug 18;436(7053):1030–1034. doi: 10.1038/nature03894

Figure 4. Failure of stem cell self-renewal causes spermatogonial depletion.

Figure 4

a–d, Loss of proliferating spermatogonia in ERM−/− testis, demonstrated by BrdU labelling in vivo of wild-type (a, c) and ERM−/− (b, d) testes at 3 weeks (a, b) and 4 weeks (c, d). e, f, Comparable levels of spermatogonial apoptosis, determined by TUNEL staining, in wild-type (e) and ERM−/− (f) testes at 4 weeks of age. Arrows indicate apoptotic cells. g, Expression of apoptosis-related genes was unchanged in ERM−/− testes as indicated by RNase protection assay of total RNA from 4-week-old wild-type (WT) and ERM−/− (KO) testes. GAPDH, glyceraldehyde3phosphate dehydrogenase. Y. RNA, Yeast tRNA.