Table 1.

DEAB rescue of cdx4^−/− embryos requires exposure during gastrulation

Stage exposed	Time exposed, hpf	Mutant embryos with increased gata1/total mutants	Mutant embryos with increased gata1, %	Degree of rescue
None	N/A	0/23	0	None
50%-90*	5.5-9	12/19	63	2+
50%-1 ss	5.5-10.3	23/24	96	2+
90%-1 ss*	9-10.3	18/21	86	1+
1 ss-5 ss*	10.3-11.7	2/25	8	Scant
50%-5 ss	5.5-11.7	21/23	91	3+
60%-10 ss*	6-14	8/8	100	3+
90%-5 ss	9-11.7	9/9	100	2+
90%-10 ss	9-14	10/10	100	1+
5 ss-10 ss*	11.7-14	0/6	0	None

Embryos from incrosses of cdx4^+/− heterozygotes were treated with DMSO or DEAB at various stages of early development. (5.5 hpf = 50% epiboly, 6 hpf = 60% epiboly, 9 hpf = 90% epiboly, 10.3 hpf = 1 ss, 11.7 hpf = 5 ss, and 14 hpf = 15 ss) The total number of mutant embryos in 2- to 3-treated groups were pooled for each incubation period. Embryos were incubated in DEAB (2 × 10⁻⁵M) and compared with control embryos incubated in DMSO to evaluate the degree of gata1 rescue. Each data point represented pooling of several (2-4) experiments. The degree of rescue was scored based on the number of gata1⁺ cells present in comparison to control embryos for that experiment. A full rescue was scored as 3+ and reflected a maximal increase of gata1⁺ cells as depicted in Figure 4D. When only a few more cells were present in comparison to the vehicle-treated control embryos, these embryos were scored as 1+ rescue. The level of rescue was deemed intermediate or 2+ if the number of gata1⁺ cells was less than maximal, or if there were mixed levels of rescue among the total number of mutant embryos scored.

N/A indicates not applicable.

^*Treatment groups depicted in Figure 5.