Fig. 2.
Effect of lactacystin upon PMA/Io-stimulated TH1 cytokine production in activated Jurkat T cells and primary splenocytes. Jurkat T cells (5 × 105 cells/ml) and murine splenocytes (5 × 106 cells/ml) were treated with lactacystin (1–10 µM) or vehicle (VH, 0.1% ethanol) for 30 min followed by activation of the cells with 40 nM/0.5 µM PMA/ionomycin (PMA/Io). Cells were harvested 6h later, the RNA isolated, and analyzed for either A.) IL-2 or B.) IFNγ. Cellular viability was ≥ 80% for all treatment groups as assessed by trypan blue exclusion. The results are presented as fold induction over BKG and are the mean ± standard error of triplicate cultures. * denotes p<0.05 compared to the VH group as determined by two-tailed Dunnett’s analysis.