Abstract
Atypical fermentation in the two-tube method of Hugh and Leifson (1953), whereby acid was produced in the sealed tube but not in the open tube, was determined to be the result of a preferential metabolism of peptone over the reactant carbohydrate. These atypical fermentations are only partially detected by one-tube oxidation-fermentation methods, and as such they constitute a source of limitation to the routine use of these abbreviated techniques for oxidative-fermentation determinations.
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Selected References
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- Brown W. J. One-tube test for determining oxidative or fermentative metabolism of bacteria. Appl Microbiol. 1974 Apr;27(4):811–813. doi: 10.1128/am.27.4.811-813.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans J. B., Kloos W. E. Use of shake cultures in a semisolid thioglycolate medium for differentiating staphylococci from micrococci. Appl Microbiol. 1972 Feb;23(2):326–331. doi: 10.1128/am.23.2.326-331.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HUGH R., LEIFSON E. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol. 1953 Jul;66(1):24–26. doi: 10.1128/jb.66.1.24-26.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hugh R. A comparison of the neotype strain and 119 isolates of Vibrio eltor Pribram 1933. Indian J Med Res. 1966 Sep;54(9):839–848. [PubMed] [Google Scholar]
- MOSSEL D. A., MARTIN G. [Simplified medium permitting study of the various modes of action of bacteria on carbohydrates]. Ann Inst Pasteur Lille. 1961;12:225–226. [PubMed] [Google Scholar]
- Porres J. M., Stanyon R. E. One-tube oxidation--fermentation test. Am J Clin Pathol. 1974 Mar;61(3):368–374. doi: 10.1093/ajcp/61.3.368. [DOI] [PubMed] [Google Scholar]
- Snell J. J., Lapage S. P. Comparison of four methods for demonstrating glucose breakdown by bacteria. J Gen Microbiol. 1971 Oct;68(2):221–225. doi: 10.1099/00221287-68-2-221. [DOI] [PubMed] [Google Scholar]