Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1976 May;31(5):784–786. doi: 10.1128/aem.31.5.784-786.1976

Lecithin agar for detection of microbial phospholipases.

G L Chrisope, C W Fox, R T Marshall
PMCID: PMC291196  PMID: 818960

Abstract

Lecithin agar was developed on which phospholipase C produced turbid zones and phospholipase A produced clear zones. Reactions on lecithin agar agreed 74% of the time with reactions in egg yolk broth. On lecithin agar, interpretation was easier, phospholipase A was detectable, and opaque zones were visible 1 or 2 days earlier than on egg yolk agar. All constituents of the medium can be autoclaved.

Full text

PDF
784

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Habermann E., Hardt K. L. A sensitive and specific plate test for the quantitation of phospholipases. Anal Biochem. 1972 Nov;50(1):163–173. doi: 10.1016/0003-2697(72)90495-2. [DOI] [PubMed] [Google Scholar]
  3. McMurray W. C., Magee W. L. Phospholipid metabolism. Annu Rev Biochem. 1972;41(10):129–160. doi: 10.1146/annurev.bi.41.070172.001021. [DOI] [PubMed] [Google Scholar]
  4. Van Heyningen W. E. The biochemistry of the gas gangrene toxins: Estimation of the alpha toxin of Cl. welchii, type A. Biochem J. 1941 Nov;35(10-11):1246–1256. doi: 10.1042/bj0351246. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES