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. Author manuscript; available in PMC: 2010 Jul 29.
Published in final edited form as: Immunogenetics. 2008 Mar 11;60(5):257–265. doi: 10.1007/s00251-008-0285-3

Figure 5. Phylogenetic analyses of NITR V domains from multiple species.

Figure 5

Neighbor-joining tree of V domains encoded by NITR genes in zebrafish (Danio rerio, “Dr”); channel catfish (Ictalurus punctatus, “Ip”); rainbow trout (Oncorhynchus mykiss, “Om”); Southern pufferfish (Sphoeroides nephelus, “Sn”); and Japanese flounder (Paralichthys olivaceus, “Po”). Protein symbols are abbreviated to the NITR number (e.g. zebrafish Nitr14a is shown as “Dr 14a”). In the instances of NITR gene families, only one member was included in the analysis. For example Sn 16 represents Sn 16 and 22; Sn 13 represents Sn 13 and 18; Sn 20 represents Sn 14, 15, 17, 19, 20, 21, 23-26; Ip 1 represents Ip 1 and 3; and Ip 5 represents Ip 5-11. The number assigned to each interior branch is the bootstrap value; bootstrap values less than 50 are not shown. The branch lengths are measured in terms of the number of amino acid substitutions estimated by Poisson correction, with the scale given below the tree. Note that zebrafish Nitr13 and Nitr14 V domains group with catfish IpNITR13 and 15 V domains (gray shading): these 4 V domains also group together when using maximal parsimony and UPGMA methods (not shown).