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. 1978 Aug;36(2):270–273. doi: 10.1128/aem.36.2.270-273.1978

Inhibition of aflatoxin biosynthesis by tolnaftate.

S N Khan, K K Maggon, T A Venkitasubramanian
PMCID: PMC291213  PMID: 697362

Abstract

Tolnaftate [2-napthyl-N-methyl-N-(m-tolyl)thionocarbamate], an antifungal drug, is widely used to control superficial fungal infections in humans and other animals. In this study the effect of tolnaftate on aflatoxin biosynthesis by Aspergillus parasiticus NRRL 3240 was investigated. Tolnaftate changed the morphology of A. parasiticus to yeastlike forms and inhibited aflatoxin formation. The formation of aflatoxin G was blocked considerably, indicating a metabolic block in the conversion of aflatoxin B to aflatoxin G. The incorporation of [1-14C]acetate into aflatoxin was significantly inhibited at a concentration of 1 mM tolnaftate. The presence of zinc in the resuspension buffer resulted in reversal of the tolnaftate-induced inhibition of aflatoxin G1 biosynthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADYE J., MATELES R. I. INCORPORATION OF LABELLED COMPOUNDS INTO AFLATOXINS. Biochim Biophys Acta. 1964 May 11;86:418–420. doi: 10.1016/0304-4165(64)90077-7. [DOI] [PubMed] [Google Scholar]
  2. Bean G. A., Rambo G. W. Use of dimethyl sulfoxide to control aflatoxin production. Ann N Y Acad Sci. 1975 Jan 27;243:237–245. doi: 10.1111/j.1749-6632.1975.tb25362.x. [DOI] [PubMed] [Google Scholar]
  3. Detroy R. W., Ciegler A. Induction of yeastlike development in Aspergillus parasiticus. J Gen Microbiol. 1971 Mar;65(3):259–264. doi: 10.1099/00221287-65-3-259. [DOI] [PubMed] [Google Scholar]
  4. Detroy R. W., Freer S., Ciegler A. Aflatoxin and anthraquinone biosynthesis by nitrosoguanidine-derived mutants of Aspergillus parasiticus. Can J Microbiol. 1973 Nov;19(11):1373–1378. doi: 10.1139/m73-221. [DOI] [PubMed] [Google Scholar]
  5. Detroy R. W., Hesseltine C. W. Secondary biosynthesis of aflatoxin B in Aspergillus parasiticus. Can J Microbiol. 1970 Oct;16(10):959–963. doi: 10.1139/m70-164. [DOI] [PubMed] [Google Scholar]
  6. Gupta S. K., Maggon K. K., Venkitasubramanian T. A. Effect of zinc on adenine nucleotide pools in relation to aflatoxin biosynthesis in Aspergillus parasiticus. Appl Environ Microbiol. 1976 Dec;32(6):753–756. doi: 10.1128/aem.32.6.753-756.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gupta S. K., Maggon K. K., Venkitasubramanian T. A. Inhibition of aflatoxin formation by 2-mercaptoethanol. Appl Environ Microbiol. 1976 Sep;32(3):324–326. doi: 10.1128/aem.32.3.324-326.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gupta S. K., Venkitasubramanian T. A. Production of aflatoxin on soybeans. Appl Microbiol. 1975 Jun;29(6):834–836. doi: 10.1128/am.29.6.834-836.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gupta S. K., Venkitasubramanian T. A. The effect of zinc and phytic acid on the incorporation of 1-14C-acetate into aflatoxin by resting mycelia of Aspergillus parasiticus. Z Lebensm Unters Forsch. 1975 Oct 31;159(2):107–111. doi: 10.1007/BF01135786. [DOI] [PubMed] [Google Scholar]
  10. Gupta S. R., Prasanna H. R., Viswanathan L., Venkitasubramanian T. A. Effect of some inhibitors on aflatoxin-production in a synthetic medium and on the incorporation of acetate-1- 14C into aflatoxins by resting mycelia of Aspergillus parasiticus. Bull Environ Contam Toxicol. 1976 Apr;15(4):447–453. doi: 10.1007/BF01685070. [DOI] [PubMed] [Google Scholar]
  11. Gupta S. R., Prasanna H. R., Viswanathan L., Venkitasubramanian T. A. Factors influencing the biosynthesis of aflatoxins. Indian J Biochem Biophys. 1975 Jun;12(2):179–181. [PubMed] [Google Scholar]
  12. Gupta S. R., Prasanna H. R., Viswanathan L., Venkitasubramanian T. A. Synthesis of aflatoxins by the non-growing mycelia of Aspergillus parasiticus and the effect of inhibitors. J Gen Microbiol. 1975 Dec;91(2):417–420. doi: 10.1099/00221287-91-2-417. [DOI] [PubMed] [Google Scholar]
  13. Gupta S. R., Prasanna H. R., Viswanathan L., Venkitasubramanian T. A. The effect of inorganic salts and some biologically important compounds on the incorporation of 1-14C acetate into aflatoxins by resting mycelia of Aspergillus parasiticus. Z Lebensm Unters Forsch. 1975 Feb 7;157(1):19–22. doi: 10.1007/BF01785723. [DOI] [PubMed] [Google Scholar]
  14. Hsieh D. P. Inhibition of aflatoxin biosynthesis of dichlorvos. J Agric Food Chem. 1973 May-Jun;21(3):468–470. doi: 10.1021/jf60187a035. [DOI] [PubMed] [Google Scholar]
  15. Kobayashi G. S., Medoff G. Antifungal agents: recent developments. Annu Rev Microbiol. 1977;31:291–308. doi: 10.1146/annurev.mi.31.100177.001451. [DOI] [PubMed] [Google Scholar]
  16. Maggon K. K., Gupta S. K., Venkitasubramanian T. A. Biosynthesis of aflatoxins. Bacteriol Rev. 1977 Dec;41(4):822–855. doi: 10.1128/br.41.4.822-855.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Maggon K. K., Venkitasubramanian T. A. Metabolism of aflatoxins B1 and G1 by Aspergillus parasiticus. Experientia. 1973 Oct 15;29(10):1210–1211. doi: 10.1007/BF01935075. [DOI] [PubMed] [Google Scholar]
  18. Reddy T. V., Viswanathan L., Venkitasubramanian T. A. High aflatoxin production on a chemically defined medium. Appl Microbiol. 1971 Sep;22(3):393–396. doi: 10.1128/am.22.3.393-396.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schroeder H. W., Cole R. J., Grigsby R. D., Hein H., Jr Inhibition of aflatoxin production and tentative identification of an aflatoxin intermediate "versiconal acetate" from treatment with dichlorvos. Appl Microbiol. 1974 Feb;27(2):394–399. doi: 10.1128/am.27.2.394-399.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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