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. 1979 Dec;38(6):1086–1091. doi: 10.1128/aem.38.6.1086-1091.1979

Occurrence and Characterization of a Phosphoenolpyruvate: Glucose Phosphotransferase System in a Marine Bacterium, Serratia marinorubra

Robert E Hodson 1, Farooq Azam 2
PMCID: PMC291250  PMID: 16345474

Abstract

The mechanism of d-glucose transport in the marine bacterium Serratia marinorubra was investigated. Uptake is mediated by a single, constitutive phosphoenolpyruvate:sugar phosphotransferase system (PTS), resulting in phosphorylation of d-glucose to d-glucose phosphate during transport. The system is saturable (Km = 6.4 × 10−6 M) and highly temperature dependent, with a Q10 of 3.5 between 5 and 15°C. The system is highly specific for d-glucose; structurally related sugars and sugar alcohols did not significantly compete with d-glucose for transport. The PTS requires Mg2+ (Km = 2.5 × 10−4 M), but its activity is otherwise unaffected by salinity changes over the range tested (0 to 35 ‰). S. marinorubra differs from other gram-negative organisms (Escherichia coli and Salmonella typhimurium) in that its glycerol (non-PTS substrate) permease is not regulated by the presence of glucose (PTS substrate).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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