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. 1980 Jan;39(1):253–260. doi: 10.1128/aem.39.1.253-260.1980

Technique for determining total bacterial virus counts in complex aqueous systems.

D L Ewert, M J Paynter
PMCID: PMC291311  PMID: 6986849

Abstract

A direct method is described for measuring bacteriophage concentrations in complex aqueous systems. Conditions for sample clarification, phage recognition, and recovery were optimized. In contrast to the plaque assay, this procedure permits quantitation of total numbers of phages independent of bacterial host. Also, the modifications increase the sensitivity of the sedimentation assay, permitting detection of particles at a minimum concentration of 10(4) per ml. Maximal total phage concentrations in the aqueous phase of sewage and activated sludge mixed liquor were 1.3 x 10(6) and 4.3 x 10(7) per ml, respectively.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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