Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1980 Feb;39(2):387–393. doi: 10.1128/aem.39.2.387-393.1980

Kinetics of Growth and Amylase Production of Saccharomycopsis fibuligera on Potato Processing Wastewater †

S A Lemmel 1, R C Heimsch 1, R A Korus 2
PMCID: PMC291342  PMID: 16345512

Abstract

The kinetics of growth and amylase production of Saccharomycopsis fibuligera were studied in a chemostat on a synthetic potato processing blancher water. Dilution rates (D) from 0.101 to 0.480 h−1 were examined. A mathematical model based on the Monod equation was developed. The yield of cell mass from carbohydrates was constant and equal to 0.84. The maximum specific growth rate and the Monod constant were determined to be 0.596 h−1 and 0.226 mg/ml, respectively. An equation for the steady-state starch concentrations was empirically derived. The steady-state noncarbohydrate carbon levels rose linearly with D. Reducing sugars were the growth-limiting substrate, and their steady-state levels conformed to Monod kinetics. The yield of amylase from the cell mass (Yz) declined as D rose and was described by the equation Yz = (−8.005D + 4.076). The model predicted that the maximum production of cell mass should occur at D = 0.35 h−1 and the maximum production of amylase should occur at D = 0.22 h−1. The mathematical model presented agreed with the experimental results in its prediction of the steady-state level of reducing sugar, starch, cell mass, and amylase concentrations as well as the productivity of amylase.

Full text

PDF
387

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cleland W. W. The statistical analysis of enzyme kinetic data. Adv Enzymol Relat Areas Mol Biol. 1967;29:1–32. doi: 10.1002/9780470122747.ch1. [DOI] [PubMed] [Google Scholar]
  2. Ebertová H. Amylolytic enzymes of Endomycopsis capsularis. I. Formation of the amylolytic system in cultures of Endomycopsis capsularis. Folia Microbiol (Praha) 1966;11(1):14–20. doi: 10.1007/BF02877150. [DOI] [PubMed] [Google Scholar]
  3. HERBERT D., ELSWORTH R., TELLING R. C. The continuous culture of bacteria; a theoretical and experimental study. J Gen Microbiol. 1956 Jul;14(3):601–622. doi: 10.1099/00221287-14-3-601. [DOI] [PubMed] [Google Scholar]
  4. Harder W., Kuenen J. G. A review. Microbial selection in continuous culture. J Appl Bacteriol. 1977 Aug;43(1):1–24. doi: 10.1111/j.1365-2672.1977.tb00717.x. [DOI] [PubMed] [Google Scholar]
  5. Lemmel S. A., Heimsch R. C., Edwards L. L. Optimizing the continuous production of Candida utilis and Saccharomycopsis fibuliger on potato processing wastewater. Appl Environ Microbiol. 1979 Feb;37(2):227–232. doi: 10.1128/aem.37.2.227-232.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Polívka L., Zelinka J. The yeast biomass production by Endomycopsis fibuliger on media with wastes of starch productions. Biologia (Bratisl) 1969;24(12):881–884. [PubMed] [Google Scholar]
  7. Polívka L., Zelinka J. The yeast biomass production of Endomycopsis fibuliger on starch media. Biologia (Bratisl) 1969;24(12):873–880. [PubMed] [Google Scholar]
  8. Stepanov A. I., Afanas-eva V. P., Zaitseva G. V., Mednikova A. P., Lupandina I. B. Reguliatsiia biosinteza fermentov amiloliticheskogo kompleksa Endomycopsis fibuligera shtamm 20-9. Prikl Biokhim Mikrobiol. 1975 Sep-Oct;11(5):682–685. [PubMed] [Google Scholar]
  9. Volkova L. D., Egorov N. S., Iarovenko V. L. Nekotorye voprosy reguliatsii sinteza gliukoamilazy u Endomycopsis fibuligera 21. Prikl Biokhim Mikrobiol. 1978 May-Jun;14(3):333–340. [PubMed] [Google Scholar]
  10. Wickerham L. J., Lockwood L. B., Pettijohn O. G., Ward G. E. Starch Hydrolysis and Fermentation by the Yeast Endomycopsis Fibuliger. J Bacteriol. 1944 Oct;48(4):413–427. doi: 10.1128/jb.48.4.413-427.1944. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES