Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1980 Feb;39(2):460–462. doi: 10.1128/aem.39.2.460-462.1980

Use of Two-Dimensional Polyacrylamide Electrophoresis to Demonstrate that Putative Rhizobium Cross-Inoculation Mutants Actually Are Contaminants

Walter T Leps 1, G P Roberts 1,, Winston J Brill 1
PMCID: PMC291354  PMID: 16345520

Abstract

Two-dimensional polyacrylamide electrophoresis was used to determine that mutants of Rhizobium trifolii DT6, claimed to be capable of effectively nodulating soybeans, were actually Rhizobium japonicum 110 contaminants isolated from the parent DT6 culture.

Full text

PDF
460

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ludwig R. A., Raleigh E. A., Duncan M. J., Signer E. R., Gibson A. H., Dudman W. F., Schwinghamer E. A., Jordan D. C., Schmidt E. L., Tran D. T. Further examination of presumptive Rhizobium trifolii mutants that nodulate Glycine max. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3942–3946. doi: 10.1073/pnas.76.8.3942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Mielenz J. R., Jackson L. E., O'Gara F., Shanmugam K. T. Fingerprinting bacterial chromosomal DNA with restriction endonuclease EcoRI: comparison of Rhizobium spp. and identification of mutants. Can J Microbiol. 1979 Jul;25(7):803–807. doi: 10.1139/m79-118. [DOI] [PubMed] [Google Scholar]
  3. O'Gara F., Shanmugam K. T. Regulation of nitrogen fixation by Rhizobia. Export of fixed N2 as NH+4. Biochim Biophys Acta. 1976 Jul 21;437(2):313–321. doi: 10.1016/0304-4165(76)90001-5. [DOI] [PubMed] [Google Scholar]
  4. O'Gara F., Shanmugam K. T. Regulation of nitrogen fixation in Rhizobium spp. Isolation of mutants of Rhizobium trifolii which induce nitrogenase activity. Biochim Biophys Acta. 1977 Dec 22;500(2):277–290. doi: 10.1016/0304-4165(77)90020-4. [DOI] [PubMed] [Google Scholar]
  5. O'gara F., Shanmugam K. T. Mutant strains of clover rhizobium (Rhizobium trifolii) that form nodules on soybean (Glycine max). Proc Natl Acad Sci U S A. 1978 May;75(5):2343–2347. doi: 10.1073/pnas.75.5.2343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Roberts G. P., Leps W. T., Silver L. E., Brill W. J. Use of two-dimensional polyacrylamide gel electrophoresis to identify and classify Rhizobium strains. Appl Environ Microbiol. 1980 Feb;39(2):414–422. doi: 10.1128/aem.39.2.414-422.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES