Abstract
A density centrifugation procedure has been developed as a replacement for soil flocculation and clarification steps employed in quantitative fluorescent-antibody studies on Rhizobium in soils. Near-quantitative recovery of added cells of two strains of Rhizobium japonicum and two strains of R. phaseoli was achieved from six soils with various properties. It is proposed that this technique may prove useful in separating other soil microorganisms from soil particles in ecological studies employing fluorescent-antibody techniques.
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Selected References
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- Bohlool B. B., Brock T. D. Immunofluorescence approach to the study of the ecology of Thermoplasma acidophilum in coal refuse material. Appl Microbiol. 1974 Jul;28(1):11–16. doi: 10.1128/am.28.1.11-16.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cole M. A., Elkan G. H. Transmissible resistance to penicillin G, neomycin, and chloramphenicol in Rhizobium japonicum. Antimicrob Agents Chemother. 1973 Sep;4(3):248–253. doi: 10.1128/aac.4.3.248. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fliermans C. B., Schmidt E. L. Autoradiography and immunofluorescence combined for autecological study of single cell activity with Nitrobacter as a model system. Appl Microbiol. 1975 Oct;30(4):676–684. doi: 10.1128/am.30.4.676-684.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hobbie J. E., Daley R. J., Jasper S. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol. 1977 May;33(5):1225–1228. doi: 10.1128/aem.33.5.1225-1228.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reyes V. G., Schmidt E. L. Population Densities of Rhizobium japonicum Strain 123 Estimated Directly in Soil and Rhizospheres. Appl Environ Microbiol. 1979 May;37(5):854–858. doi: 10.1128/aem.37.5.854-858.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strayer R. F., Tiedje J. M. Application of the fluorescent-antibody technique to the study of a methanogenic bacterium in lake sediments. Appl Environ Microbiol. 1978 Jan;35(1):192–198. doi: 10.1128/aem.35.1.192-198.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]