Abstract
Two predominant rumen cellulolytic bacteria, Ruminococcus flavefaciens C94 and Bacteroides succinogenes S85, were incubated with ground filter paper (Whatman no. 1), cattle manure fiber, wheat straw, Kentucky bluegrass, alfalfa, and corn silage as substrates. Analyses of the initial substrate and the recovered residue after 48 h of static incubation showed that R. flavefaciens C94 was quantitatively more effective than B. succinogenes S85 in degrading total dry matter (32.3% versus 16.1%). However, B. succinogenes S85 demonstrated a qualitative advantage in degrading the hemicellulose and hemicellulosic sugars of particular substrates. R. flavefaciens degraded a mean 29.7% of the cellulose and 35.6% of the hemicellulose in the various substrates, whereas B. succinogenes degraded a mean 17.9 and 31.6% of these fractions, respectively. Gas-liquid chromatography was an important aid in characterizing the polysaccharide-degrading capabilities of these rumen species.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BRYANT M. P., ROBINSON I. M. Some nutritional characteristics of predominant culturable ruminal bacteria. J Bacteriol. 1962 Oct;84:605–614. doi: 10.1128/jb.84.4.605-614.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BRYANT M. P., SMALL N., BOUMA C., ROBINSON I. M. Characteristics of ruminal anaerobic celluloytic cocci and Cillobacterium cellulosolvens n. sp. J Bacteriol. 1958 Nov;76(5):529–537. doi: 10.1128/jb.76.5.529-537.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bryant M. P. Commentary on the Hungate technique for culture of anaerobic bacteria. Am J Clin Nutr. 1972 Dec;25(12):1324–1328. doi: 10.1093/ajcn/25.12.1324. [DOI] [PubMed] [Google Scholar]
- Caldwell D. R., Bryant M. P. Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria. Appl Microbiol. 1966 Sep;14(5):794–801. doi: 10.1128/am.14.5.794-801.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coen J. A., Dehority B. A. Degradation and utilization of hemicellulose from intact forages by pure cultures of rumen bacteria. Appl Microbiol. 1970 Sep;20(3):362–368. doi: 10.1128/am.20.3.362-368.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DEHORITY B. A. DEGRADATION AND UTILIZATION OF ISOLATED HEMICELLULOSE BY PURE CULTURES OF CELLULOLYTIC RUMEN BACTERIA. J Bacteriol. 1965 Jun;89:1515–1520. doi: 10.1128/jb.89.6.1515-1520.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dehority B. A. Pectin-fermenting bacteria isolated from the bovine rumen. J Bacteriol. 1969 Jul;99(1):189–196. doi: 10.1128/jb.99.1.189-196.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HALLIWELL G., BRYANT M. P. THE CELLULOLYTIC ACTIVITY OF PURE STRAINS OF BACTERIA FROM THE RUMEN OF CATTLE. J Gen Microbiol. 1963 Sep;32:441–448. doi: 10.1099/00221287-32-3-441. [DOI] [PubMed] [Google Scholar]
- HUNGATE R. E. The anaerobic mesophilic cellulolytic bacteria. Bacteriol Rev. 1950 Mar;14(1):1–49. doi: 10.1128/br.14.1.1-49.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Latham M. J., Brooker B. E., Pettipher G. L., Harris P. J. Adhesion of Bacteroides succinogenes in pure culture and in the presence of Ruminococcus flavefaciens to cell walls in leaves of perennial ryegrass (Lolium perenne). Appl Environ Microbiol. 1978 Jun;35(6):1166–1173. doi: 10.1128/aem.35.6.1166-1173.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Latham M. J., Brooker B. E., Pettipher G. L., Harris P. J. Ruminococcus flavefaciens Cell Coat and Adhesion to Cotton Cellulose and to Cell Walls in Leaves of Perennial Ryegrass (Lolium perenne). Appl Environ Microbiol. 1978 Jan;35(1):156–165. doi: 10.1128/aem.35.1.156-165.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sloneker J. H. Determination of cellulose and apparent hemicellulose in plant tissue by gas-liquid chromatography. Anal Biochem. 1971 Oct;43(2):539–546. doi: 10.1016/0003-2697(71)90285-5. [DOI] [PubMed] [Google Scholar]