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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Neuroscience. 2010 Jun 9;169(3):1085–1093. doi: 10.1016/j.neuroscience.2010.04.080

Figure 6.

Figure 6

Immunoblot and densitometric analysis of striatal TH for mice exposed in utero to PBS (blocks 1, 3, 5 and 7) or 10 mg/kg MPTP (blocks 2, 4, 6, and 8). The mice were treated postnatal at 12 weeks with PBS or 10, 20 or 30 mg/kg of MPTP. The bands indicate the quantities of TH proteins for the 4 treatment pairs. Postnatal MPTP caused dose-related reduction of TH that was more severe in the offspring that were exposed prenatally to 10 mg/kg of MPTP (columns 2, 4, 6 and 8, as compared to the matching prenatal PBS-exposed group (columns 1, 3, 5 and 7). Whereas, the 10 mg/kg of prenatal MPTP exposure reduced TH by 38 % (block 2 vs 1), the postnatal 10 mg/kg of MPTP reduced TH by only 9.4% (block 3 vs 1), which shows that the fetus is vulnerable to the MPTP toxin as compared to the 12 weeks offspring. TH density was expressed as TH/β-actin ratio with reference to total protein.