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. 1980 Apr;39(4):877–881. doi: 10.1128/aem.39.4.877-881.1980

Tracer Analysis of Methanogenesis in Salt Marsh Soils †

Gary M King 1,‡, W J Wiebe 1,§
PMCID: PMC291437  PMID: 16345551

Abstract

Differences in paths of carbon flow have been found in soils of the tall (TS) and short (SS) Spartina alterniflora marshes of Sapelo Island, Ga. Gaseous end products of [U-14C]glucose metabolism were 14CO2 and 14CH4 in the SS region and primarily 14CO2 in the TS region. Sulfate concentration did not demonstrably affect glucose catabolism or the distribution of end products in either zone. [U-14C]acetate was converted to 14CO2 and 14CH4 in the SS soils and almost exclusively to 14CO2 in the TS soils. Sulfate concentration did not affect acetate metabolism in the SS soils; however, a noticeable effect of sulfate dilution was seen in TS soils. Sulfate dilution in TS samples resulted in increased methane formation. Total glucose and acetate metabolism were similar in TS and SS soils despite differences in end products. A microbial community characterized by fermentative/sulfate-reducing processes has developed in TS soils as opposed to the fermentative/methanogenic/sulfate-reducing community found in SS soils.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abram J. W., Nedwell D. B. Hydrogen as a substrate for methanogenesis and sulphate reduction in anaerobic saltmarsh sediment. Arch Microbiol. 1978 Apr 27;117(1):93–97. doi: 10.1007/BF00689357. [DOI] [PubMed] [Google Scholar]
  2. Abram J. W., Nedwell D. B. Inhibition of methanogenesis by sulphate reducing bacteria competing for transferred hydrogen. Arch Microbiol. 1978 Apr 27;117(1):89–92. doi: 10.1007/BF00689356. [DOI] [PubMed] [Google Scholar]
  3. Badziong W., Thauer R. K. Growth yields and growth rates of Desulfovibrio vulgaris (Marburg) growing on hydrogen plus sulfate and hydrogen plus thiosulfate as the sole energy sources. Arch Microbiol. 1978 May 30;117(2):209–214. doi: 10.1007/BF00402310. [DOI] [PubMed] [Google Scholar]
  4. Badziong W., Thauer R. K., Zeikus J. G. Isolation and characterization of Desulfovibrio growing on hydrogen plus sulfate as the sole energy source. Arch Microbiol. 1978 Jan 23;116(1):41–49. doi: 10.1007/BF00408732. [DOI] [PubMed] [Google Scholar]
  5. Bryant M. P., Campbell L. L., Reddy C. A., Crabill M. R. Growth of desulfovibrio in lactate or ethanol media low in sulfate in association with H2-utilizing methanogenic bacteria. Appl Environ Microbiol. 1977 May;33(5):1162–1169. doi: 10.1128/aem.33.5.1162-1169.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cappenberg T. E. Interrelations between sulfate-reducing and methane-producing bacteria in bottom deposits of a fresh-water lake. I. Field observations. Antonie Van Leeuwenhoek. 1974;40(2):285–295. doi: 10.1007/BF00394387. [DOI] [PubMed] [Google Scholar]
  7. Cappenberg T. E., Prins R. A. Interrelations between sulfate-reducing and methane-producing bacteria in bottom deposits of a fresh-water lake. 3. Experiments with 14C-labeled substrates. Antonie Van Leeuwenhoek. 1974;40(3):457–469. doi: 10.1007/BF00399358. [DOI] [PubMed] [Google Scholar]
  8. Sherr B. F., Payne W. J. Effect of the Spartina alterniflora Root-Rhizome System on Salt Marsh Soil Denitrifying Bacteria. Appl Environ Microbiol. 1978 Apr;35(4):724–729. doi: 10.1128/aem.35.4.724-729.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sorokin Y. I. Role of carbon dioxide and acetate in biosynthesis by sulphate-reducing bacteria. Nature. 1966 Apr 30;210(5035):551–552. doi: 10.1038/210551a0. [DOI] [PubMed] [Google Scholar]
  10. Strayer R. F., Tiedje J. M. Kinetic parameters of the conversion of methane precursors to methane in a hypereutrophic lake sediment. Appl Environ Microbiol. 1978 Aug;36(2):330–340. doi: 10.1128/aem.36.2.330-340.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Widdel F., Pfennig N. A new anaerobic, sporing, acetate-oxidizing, sulfate-reducing bacterium, Desulfotomaculum (emend.) acetoxidans. Arch Microbiol. 1977 Feb 4;112(1):119–122. doi: 10.1007/BF00446665. [DOI] [PubMed] [Google Scholar]
  12. Winfrey M. R., Zeikus J. G. Effect of sulfate on carbon and electron flow during microbial methanogenesis in freshwater sediments. Appl Environ Microbiol. 1977 Feb;33(2):275–281. doi: 10.1128/aem.33.2.275-281.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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