Abstract
A polyacrylamide slab gel electrophoresis procedure was used to compare cellular proteins from bacterial isolates of gingival crevice floras. Isolates with identical protein patterns consistently were shown to be members of the same species. When used to screen isolates, the procedure reduced total analytical time and expense without sacrificing accuracy, and it provided additional verification of the identity of strains characterized by conventional phenotypic tests.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Allen R. C. Applications of polyacrylamide gel electrophoresis and polyacrylamide gel isoelectric focusing in clinical chemistry. J Chromatogr. 1978 Jul 1;146(1):1–32. doi: 10.1016/s0378-4347(00)81286-4. [DOI] [PubMed] [Google Scholar]
- Ames G. F. Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Membrane, soluble, and periplasmic fractions. J Biol Chem. 1974 Jan 25;249(2):634–644. [PubMed] [Google Scholar]
- DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
- Gross C. S., Ferguson D. A., Jr, Cummins C. S. Electrophoretic protein patterns and enzyme mobilities in anaerobic coryneforms. Appl Environ Microbiol. 1978 Jun;35(6):1102–1108. doi: 10.1128/aem.35.6.1102-1108.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jarvis A. W., Wolff J. M. Grouping of lactic streptococci by gel electrophoresis of soluble cell extracts. Appl Environ Microbiol. 1979 Mar;37(3):391–398. doi: 10.1128/aem.37.3.391-398.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kersters K., De Ley J. Identification and grouping of bacteria by numerical analysis of their electrophoretic protein patterns. J Gen Microbiol. 1975 Apr;87(2):333–342. doi: 10.1099/00221287-87-2-333. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- MCCOLLISTER D. D., OYEN F., ROWE V. K. TOXICOLOGY OF ACRYLAMIDE. Ther Ggw. 1964 Mar;103:172–181. doi: 10.1016/0041-008x(64)90103-6. [DOI] [PubMed] [Google Scholar]
- O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
- ORNSTEIN L. DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY. Ann N Y Acad Sci. 1964 Dec 28;121:321–349. doi: 10.1111/j.1749-6632.1964.tb14207.x. [DOI] [PubMed] [Google Scholar]
- Strom A., Dyer J. K., Marsh C., Tribble J. L. Identification and characterization of species of the family Bacteriodaceae by polyacrylamide gel electrophoresis. J Dent Res. 1976 Mar-Apr;55(2):252–256. doi: 10.1177/00220345760550021501. [DOI] [PubMed] [Google Scholar]
- Studier F. W. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. doi: 10.1016/0022-2836(73)90003-x. [DOI] [PubMed] [Google Scholar]
- Swindlehurst C. A., Shah H. N., Parr C. W., Williams R. A. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of polypeptides from Bacteroides melaninogenicus. J Appl Bacteriol. 1977 Oct;43(2):319–324. doi: 10.1111/j.1365-2672.1977.tb00757.x. [DOI] [PubMed] [Google Scholar]
- Swings J., De Ley J. The biology of Zymomonas. Bacteriol Rev. 1977 Mar;41(1):1–46. doi: 10.1128/br.41.1.1-46.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]