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. 1980 May;39(5):1070–1073. doi: 10.1128/aem.39.5.1070-1073.1980

Simple method for demonstrating small plasmid deoxyribonucleic acid molecules in oral streptococci.

F L Macrina, P H Wood, K R Jones
PMCID: PMC291477  PMID: 7396483

Abstract

A simple procedure for rapidly demonstrating small plasmids (less than 10 megadaltons) in oral streptococci is described. Logarithmic-phase, glycine-treated cells from 1.5-ml broth cultures were converted to osmotically fragile forms and lysed with sodium dodecyl sulfate. After the hydrodynamic shearing of host chromosomal deoxyribonucleic acid, such lysates were analyzed by low-voltage agarose gel electrophoresis. Small plasmids, migrating significantly faster than chromosomal deoxyribonucleic acid under such conditions, were readily visualized by ethidium bromide staining.

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Selected References

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