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. 1980 May;39(5):1078–1080. doi: 10.1128/aem.39.5.1078-1080.1980

Acetylene Reduction Assay for Nitrogenase Activity: Gas Chromatographic Determination of Ethylene Per Sample in Less Than One Minute

K A V David 1, S K Apte 1, A Banerji 1, Joseph Thomas 1
PMCID: PMC291480  PMID: 16345570

Abstract

Ammoniacal silver nitrate (10 mg/ml) was added to terminate acetylene reduction assays used to measure nitrogenase activity. Silver nitrate quantitatively precipitated acetylene as the carbide salt, but did not affect the ethylene formed. The vials containing ethylene can be analyzed gas chromatographically at the rate of about 13 samples in 10 min.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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