Abstract
The selective incorporation of precursors specific for individual fatty acids in germinating and outgrowing spores of Bacillus thuringiensis is described. The specific precursors utilized were [14C]butyrate, -isobutyrate, -valerate, and -isovalerate, which were incorporated into even-numbered normal-chain isomers, even-numbered iso-isomers, odd-numbered normal-chain acids, and odd-numbered isohomologs, respectively. This preferential incorporation by B. thuringiensis allows the terminal carbons of specific normal and branched-chain fatty acids, contained within the cytoplasmic membrane, to be labeled with 14C and, potentially, 13C.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Birdsall N. J., Ellar D. J., Lee A. G., Metcalfe J. C., Warren G. B. 13C-enriched phosphatidylethanolamines from Escherichia coli. Biochim Biophys Acta. 1975 Feb 20;380(2):344–354. doi: 10.1016/0005-2760(75)90020-x. [DOI] [PubMed] [Google Scholar]
- Bloch K. Fatty acid synthases from Mycobacterium phlei. Methods Enzymol. 1975;35:84–90. doi: 10.1016/0076-6879(75)35141-0. [DOI] [PubMed] [Google Scholar]
- Butterworth P. H., Bloch K. Comparative aspects of fatty acid synthesis in Bacillus subtilis and Escherichia coli. Eur J Biochem. 1970 Feb;12(3):496–501. doi: 10.1111/j.1432-1033.1970.tb00878.x. [DOI] [PubMed] [Google Scholar]
- Daniels M. J. Lipid synthesis in relation to the cell cycle of Bacillus megaterium KM and Escherichia coli. Biochem J. 1969 Dec;115(4):697–701. doi: 10.1042/bj1150697. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Daron H. H. Nutritional alteration of the fatty acid composition of a thermophilic Bacillus species. J Bacteriol. 1973 Dec;116(3):1096–1099. doi: 10.1128/jb.116.3.1096-1099.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ingram L. O., Chevalier L. S., Gabba E. J., Ley K. D., Winters K. Propionate-induced synthesis of odd-chain-length fatty acids by Escherichia coli. J Bacteriol. 1977 Sep;131(3):1023–1025. doi: 10.1128/jb.131.3.1023-1025.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KANEDA T. Biosynthesis of branched chain fatty acids. II. Microbial synthesis of branched long chain fatty acids from certain short chain fatty acid substrates. J Biol Chem. 1963 Apr;238:1229–1235. [PubMed] [Google Scholar]
- Kaneda T. Fatty acids of the genus Bacillus: an example of branched-chain preference. Bacteriol Rev. 1977 Jun;41(2):391–418. doi: 10.1128/br.41.2.391-418.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nickerson K. W., Bulla L. A., Jr, Mounts T. L. Lipid metabolism during bacterial growth, sporulation, and germination: differential synthesis of individual branched- and normal-chain fatty acids during spore germination and outgrowth of Bacillus thuringiensis. J Bacteriol. 1975 Dec;124(3):1256–1262. doi: 10.1128/jb.124.3.1256-1262.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nickerson K. W., De Pinto J., Bulla L. A., Jr Lipid metabolism during bacterial growth, sporulation, and germination: kinetics of fatty acid and macromolecular synthesis during spore germination and outgrowth of Bacillus thuringiensis. J Bacteriol. 1975 Jan;121(1):227–233. doi: 10.1128/jb.121.1.227-233.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scandella C. J., Kornberg A. Biochemical studies of bacterial sporulation and germination. XV. Fatty acids in growth, sporulation, and germination of Bacillus megaterium. J Bacteriol. 1969 Apr;98(1):82–86. doi: 10.1128/jb.98.1.82-86.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stoffel W., Bister K., Schreiber C., Tunggal B. 13C-NMR studies of the membrane structure of enveloped virions (vesicular stomatitis virus). Hoppe Seylers Z Physiol Chem. 1976 Jul;357(7):905–915. doi: 10.1515/bchm2.1976.357.2.905. [DOI] [PubMed] [Google Scholar]
- WEGNER G. H., FOSTER E. M. Incorporation of isobutyrate and valerate into cellular plasmalogen by Bacteroides succinogenes. J Bacteriol. 1963 Jan;85:53–61. doi: 10.1128/jb.85.1.53-61.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Willecke K., Pardee A. B. Fatty acid-requiring mutant of bacillus subtilis defective in branched chain alpha-keto acid dehydrogenase. J Biol Chem. 1971 Sep 10;246(17):5264–5272. [PubMed] [Google Scholar]