Abstract
A study was conducted to determine the effects of o-nitrobenzoate, p-aminobenzoate, benzocaine (ethyl aminobenzoate), ethyl benzoate, methyl benzoate, salicylic acid (o-hydroxybenzoate), trans-cinnamic acid (beta-phenylacrylic acid), trans-cinnamaldehyde (3-phenylpropenal), ferulic acid (p-hydroxy-3-methoxycinnamic acid), aspirin (o-acetoxy benzoic acid), and anthranilic acid (o-aminobenzoic acid) upon growth and aflatoxin release in Aspergillus flavus NRRL 3145 and A. parasiticus NRRL 3240. A chemically defined medium was supplemented with various concentrations of these compounds and inoculated with spores, and the developing cultures were incubated for 4, 6, and 8 days at 27 degree C in a mechanical shaker. At the beginning of day 8 of incubation, aflatoxins were extracted from cell-free filtrates, separated by thin-layer chromatography, and quantitated by ultraviolet spectrophotometry. The structure of these aromatic compounds appeared to be critically related to their effects on mycelial growth and aflatoxin release. At concentrations of 2.5 and 5.0 mg per 25 ml of medium, methyl benzoate and ethyl benzoate were the most effective in reducing both mycelial growth and aflatoxin release by A. flavus and A. parasiticus. Inhibition of mycelial growth and aflatoxin release by various concentrations of the above-named aromatic compounds may indicate the possibility of their use as fungicides.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ADYE J., MATELES R. I. INCORPORATION OF LABELLED COMPOUNDS INTO AFLATOXINS. Biochim Biophys Acta. 1964 May 11;86:418–420. doi: 10.1016/0304-4165(64)90077-7. [DOI] [PubMed] [Google Scholar]
- Applegate K. L., Chipley J. R. Daily variations in the production of aflatoxins by Aspergillus flavus NRRL-3145 following exposure to 60 Co irradiation. J Appl Bacteriol. 1974 Sep;37(3):359–372. doi: 10.1111/j.1365-2672.1974.tb00452.x. [DOI] [PubMed] [Google Scholar]
- Applegate K. L., Chipley J. R. Effects of 60Co gamma irradiation on aflatoxin B1 and B2 production by Aspergillus flavus. Mycologia. 1974 May-Jun;66(3):436–445. [PubMed] [Google Scholar]
- Davis N. D., Diener U. L. Inhibition of Aflatoxin Synthesis by p-Aminobenzoic Acid, Potassium Sulfite, and Potassium Fluoride. Appl Microbiol. 1967 Nov;15(6):1517–1518. doi: 10.1128/am.15.6.1517-1518.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gupta S. R., Prasanna H. R., Viswanathan L., Venkitasubramanian T. A. Synthesis of aflatoxins by the non-growing mycelia of Aspergillus parasiticus and the effect of inhibitors. J Gen Microbiol. 1975 Dec;91(2):417–420. doi: 10.1099/00221287-91-2-417. [DOI] [PubMed] [Google Scholar]
- HINES W. J., SMITH M. J. INHIBITION OF DEHYDROGENASES BY SALICYLATE. Nature. 1964 Jan 11;201:192–192. doi: 10.1038/201192a0. [DOI] [PubMed] [Google Scholar]
- Maggon K. K., Gupta S. K., Venkitasubramanian T. A. Biosynthesis of aflatoxins. Bacteriol Rev. 1977 Dec;41(4):822–855. doi: 10.1128/br.41.4.822-855.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Muller R. D., Carlson C. W., Semeniuk G., Harshfield G. S. The response of chicks, ducklings, goslings, pheasants and poults to graded levels of aflatoxins. Poult Sci. 1970 Sep;49(5):1346–1350. doi: 10.3382/ps.0491346. [DOI] [PubMed] [Google Scholar]
- Schroeder H. W., Cole R. J., Grigsby R. D., Hein H., Jr Inhibition of aflatoxin production and tentative identification of an aflatoxin intermediate "versiconal acetate" from treatment with dichlorvos. Appl Microbiol. 1974 Feb;27(2):394–399. doi: 10.1128/am.27.2.394-399.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Uraih N., Chipley J. R. Effects of various acids and salts on growth and aflatoxin production by Aspergillus flavus NRRL 3145. Microbios. 1976;17(67):51–59. [PubMed] [Google Scholar]
- Yao R. C., Hsieh D. P. Step of dichlorvos inhibition in the pathway of aflatoxin biosynthesis. Appl Microbiol. 1974 Jul;28(1):52–57. doi: 10.1128/am.28.1.52-57.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]