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. 1980 Aug;40(2):386–390. doi: 10.1128/aem.40.2.386-390.1980

Cell envelope damage in Escherichia coli caused by short-term stress in water.

S K Zaske, W S Dockins, G A McFeters
PMCID: PMC291586  PMID: 7008696

Abstract

Physiological and morphological changes in Escherichia coli exposed to oligotrophic natural waters and reagent grade water were studied. Several lines of evidence indicated that short-term exposure in water causes cellular envelope damage. Increasing susceptibility to lysozyme, lag time before cell division, and injury as defined by differential counts on selective and nonselective media occurred with exposure time. Electron micrographs of injured cells showed morphological changes in cell envelope.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bayer M. E. Response of Cell Walls of Escherichia coli to a Sudden Reduction of the Environmental Osmotic Pressure. J Bacteriol. 1967 Mar;93(3):1104–1112. doi: 10.1128/jb.93.3.1104-1112.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beuchat L. R. Injury and repair of gram-negative bacteria, with special consideration of the involvement of the cytoplasmic membrane. Adv Appl Microbiol. 1978;23:219–243. doi: 10.1016/s0065-2164(08)70071-6. [DOI] [PubMed] [Google Scholar]
  3. Bissonnette G. K., Jezeski J. J., McFeters G. A., Stuart D. G. Influence of environmental stress on enumeration of indicator bacteria from natural waters. Appl Microbiol. 1975 Feb;29(2):186–194. doi: 10.1128/am.29.2.186-194.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burman L. G., Nordström K., Bloom G. D. Murein and the outer penetration barrier of Escherichia coli K-12, Proteus mirabilis, and Pseudomonas aeruginosa. J Bacteriol. 1972 Dec;112(3):1364–1374. doi: 10.1128/jb.112.3.1364-1374.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hill M. J. Action of bile salts on bacterial cell walls. Nature. 1967 Jun 10;214(5093):1152–1154. doi: 10.1038/2141152a0. [DOI] [PubMed] [Google Scholar]
  6. Hobbie J. E., Daley R. J., Jasper S. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol. 1977 May;33(5):1225–1228. doi: 10.1128/aem.33.5.1225-1228.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hoekstra D., van der Laan J. W., de Leij L., Witholt B. Release of outer membrane fragments from normally growing Escherichia coli. Biochim Biophys Acta. 1976 Dec 14;455(3):889–899. doi: 10.1016/0005-2736(76)90058-4. [DOI] [PubMed] [Google Scholar]
  8. Knox K. W., Vesk M., Work E. Relation between excreted lipopolysaccharide complexes and surface structures of a lysine-limited culture of Escherichia coli. J Bacteriol. 1966 Oct;92(4):1206–1217. doi: 10.1128/jb.92.4.1206-1217.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Koplow J., Goldfine H. Alterations in the outer membrane of the cell envelope of heptose-deficient mutants of Escherichia coli. J Bacteriol. 1974 Feb;117(2):527–543. doi: 10.1128/jb.117.2.527-543.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MacAlister T. J., Irvin R. T., Costerton J. W. Cell envelope protection of alkaline phosphatase against acid denaturation in Escherichia coli. J Bacteriol. 1977 Apr;130(1):339–346. doi: 10.1128/jb.130.1.339-346.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McFeters G. A., Stuart D. G. Survival of coliform bacteria in natural waters: field and laboratory studies with membrane-filter chambers. Appl Microbiol. 1972 Nov;24(5):805–811. doi: 10.1128/am.24.5.805-811.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Morgan C., Rosenkranz H. S. Ultrastructure of Escherichia coli depleted of an amino acid. J Bacteriol. 1970 May;102(2):584–587. doi: 10.1128/jb.102.2.584-587.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mug-Opstelten D., Witholt B. Preferential release of new outer membrane fragments by exponentially growing Escherichia coli. Biochim Biophys Acta. 1978 Apr 4;508(2):287–295. doi: 10.1016/0005-2736(78)90331-0. [DOI] [PubMed] [Google Scholar]
  14. Ribi E., Anacker R. L., Brown R., Haskins W. T., Malmgren B., Milner K. C., Rudbach J. A. Reaction of endotoxin and surfactants. I. Physical and biological properties of endotoxin treated with sodium deoxycholate. J Bacteriol. 1966 Nov;92(5):1493–1509. doi: 10.1128/jb.92.5.1493-1509.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Scheusner D. L., Busta F. F., Speck M. L. Injury of bacteria by sanitizers. Appl Microbiol. 1971 Jan;21(1):41–45. doi: 10.1128/am.21.1.41-45.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tamaki S., Matsuhashi M. Increase in sensitivity to antibiotics and lysozyme on deletion of lipopolysaccharides in Escherichia coli strains. J Bacteriol. 1973 Apr;114(1):453–454. doi: 10.1128/jb.114.1.453-454.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wiebe W. J., Chapman G. B. Fine structure of selected marine pseudomonads and achromobacters. J Bacteriol. 1968 May;95(5):1862–1873. doi: 10.1128/jb.95.5.1862-1873.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Woldringh C. L. Lysis of the cell membrane of Escherichia coli K12 by ionic detergents. Biochim Biophys Acta. 1970 Nov 12;224(1):288–290. doi: 10.1016/0005-2787(70)90650-7. [DOI] [PubMed] [Google Scholar]
  19. Yem D. W., Wu H. C. Physiological characterization of an Escherichia coli mutant altered in the structure of murein lipoprotein. J Bacteriol. 1978 Mar;133(3):1419–1426. doi: 10.1128/jb.133.3.1419-1426.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Zaske S. K., Dockins W. S., Schillinger J. E., McFeters G. A. New methods to assess bacterial injury in water. Appl Environ Microbiol. 1980 Mar;39(3):656–658. doi: 10.1128/aem.39.3.656-658.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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