Abstract
The effect of various stages of the irradiation processing of beef on the injury and inactivation of radiation-resistant Moraxella-Acinetobactor cells was studied. Moraxella-Acinetobacter cells were more resistant to heat inactivation and injury when heated in meat with salts (0.75% NaCl and 0.375% sodium tripolyphosphate) then in meat without salts. These salts had no effect on radiation resistance. Both radiation- and heat-injured cells were unable to form colonies at 30 degrees C in plate count agar containing 0.8% NaCl. Neither unstressed nor heat-stressed cells were able to multiply in minced beef incubated at 30 degrees C for 12 h. Only after the beef was diluted 1:10 with peptone water were the heat-injured cells able to repair and eventually multiply. Heated cells were more sensitive to radiation inactivation and injury than unheated cells. After repair, the cells regained their resistance to both NaCl and irradiation. Freezing and storage at -40 degrees C for 14 days had only a slight effect on either unstressed or heat-stressed cells.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baird-Parker A. C., Boothroyd M., Jones E. The effect of water activity on the heat resistance of heat sensitive and heat resistant strains of salmonellae. J Appl Bacteriol. 1970 Sep;33(3):515–522. doi: 10.1111/j.1365-2672.1970.tb02228.x. [DOI] [PubMed] [Google Scholar]
- Briggs A., Yazdany S. Effect of sodium chloride on the heat and radiation resistance and on the recovery of heated or irradiated spores of the genus bacillus. J Appl Bacteriol. 1970 Dec;33(4):621–632. doi: 10.1111/j.1365-2672.1970.tb02243.x. [DOI] [PubMed] [Google Scholar]
- Calcott P. H., Lee S. K., MacLeod R. A. The effect of cooling and warming rates on the survival of a variety of bacteria. Can J Microbiol. 1976 Jan;22(1):106–109. doi: 10.1139/m76-015. [DOI] [PubMed] [Google Scholar]
- Calhoun C. L., Frazier W. C. Effect of available water on thermal resistance of three nonsporeforming species of bacteria. Appl Microbiol. 1966 May;14(3):416–420. doi: 10.1128/am.14.3.416-420.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Duncan C. L., Foster E. M. Role of curing agents in the preservation of shelf-stable canned meat products. Appl Microbiol. 1968 Feb;16(2):401–405. doi: 10.1128/am.16.2.401-405.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- El-Zawahry Y. A., Rowley D. B. Radiation resistance and injury of Yersinia enterocolitica. Appl Environ Microbiol. 1979 Jan;37(1):50–54. doi: 10.1128/aem.37.1.50-54.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Firstenberg-Eden R., Rowley D. B., Shattuck E. Thermal inactivation and injury of Moraxella-Acinetobacter cells in ground beef. Appl Environ Microbiol. 1980 Jan;39(1):159–164. doi: 10.1128/aem.39.1.159-164.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hariharan P. P., Cerutti P. A. Repair of gamma-ray-induced thymine damage in Micrococcus radiodurans. Nat New Biol. 1971 Feb 24;229(8):247–249. doi: 10.1038/newbio229247a0. [DOI] [PubMed] [Google Scholar]
- MAZUR P. KINETICS OF WATER LOSS FROM CELLS AT SUBZERO TEMPERATURES AND THE LIKELIHOOD OF INTRACELLULAR FREEZING. J Gen Physiol. 1963 Nov;47:347–369. doi: 10.1085/jgp.47.2.347. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Notermans S., Kampelmacher E. H. Heat destruction of some bacterial strains attached to broiler skin. Br Poult Sci. 1975 Jul;16(4):351–361. doi: 10.1080/00071667508416199. [DOI] [PubMed] [Google Scholar]
- Welch A. B., Maxcy R. B. Characterization of radiation-resistant vegetative bacteria in beef. Appl Microbiol. 1975 Aug;30(2):242–250. doi: 10.1128/am.30.2.242-250.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]