Abstract
The inactivation rates of coxsackievirus B3 (CB3) and B5 (CB5) by chlorine in dilute buffer at pH 6 were very nearly the same and about half that of poliovirus (Mahoney) under similar conditions. Purified CB3, like the poliovirus, aggregated in the acid range but not at pH 7 and above. Purified CB5 aggregated rapidly at all pH values; still, the graph of log surviving infectivity versus time was a straight line. No chlorine inactivation data were obtained with dispersed CB5, for it could be dispersed only by addition of diethylaminoethyl dextran, which would react with the chlorine. Addition of 0.1 M NaCl to the buffer at pH 6 did not influence the aggregation of CB5 or the rate of chlorine action on either of the coxsackie-viruses, but at pH 10 it increased the disinfection activity of OCl- for both viruses roughly 20-fold. Cesium chloride had a similar but smaller effect. KCl was the most active of the three in this respect, making the inactivating effect of OCl- at pH 10 about equal to that of HOCl at pH 6.
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- Floyd R., Johnson J. D., Sharp D. G. Inactivation by bromine of single poliovirus particles in water. Appl Environ Microbiol. 1976 Feb;31(2):298–303. doi: 10.1128/aem.31.2.298-303.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Floyd R., Sharp D. G. Aggregation of poliovirus and reovirus by dilution in water. Appl Environ Microbiol. 1977 Jan;33(1):159–167. doi: 10.1128/aem.33.1.159-167.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Floyd R., Sharp D. G. Viral aggregation: effects of salts on the aggregation of poliovirus and reovirus at low pH. Appl Environ Microbiol. 1978 Jun;35(6):1084–1094. doi: 10.1128/aem.35.6.1084-1094.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Floyd R., Sharp D. G. Viral aggregation: quantitation and kinetics of the aggregation of poliovirus and reovirus. Appl Environ Microbiol. 1978 Jun;35(6):1079–1083. doi: 10.1128/aem.35.6.1079-1083.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sharp D. G., Floyd R., Johnson J. D. Initial fast reaction of bromine on reovirus in turbulent flowing water. Appl Environ Microbiol. 1976 Feb;31(2):173–181. doi: 10.1128/aem.31.2.173-181.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sharp D. G., Young D. C., Floyd R., Johnson J. D. Effect of ionic environment on the inactivation of poliovirus in water by chlorine. Appl Environ Microbiol. 1980 Mar;39(3):530–534. doi: 10.1128/aem.39.3.530-534.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Totsuka A., Ohtaki K., Tagaya I. Aggregation of enterovirus small plaque variants and polioviruses under low ionic strength conditions. J Gen Virol. 1978 Mar;38(3):519–533. doi: 10.1099/0022-1317-38-3-519. [DOI] [PubMed] [Google Scholar]
- WEIDENKOPF S. J. Inactivation of type 1, poliomyelitis virus with chlorine. Virology. 1958 Feb;5(1):56–67. doi: 10.1016/0042-6822(58)90005-9. [DOI] [PubMed] [Google Scholar]
- Young D. C., Johnson J. D., Sharp D. G. The complex reaction kinetics of ECHO-1 virus with chlorine in water. Proc Soc Exp Biol Med. 1977 Dec;156(3):496–499. doi: 10.3181/00379727-156-39965. [DOI] [PubMed] [Google Scholar]
- Young D. C., Sharp D. G. Poliovirus aggregates and their survival in water. Appl Environ Microbiol. 1977 Jan;33(1):168–177. doi: 10.1128/aem.33.1.168-177.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]