Abstract
Pyrolysis gas-liquid chromatography (PGLC) was used to differentiate between HeLa cell-invasive and noninvasive strains of Yersinia enterocolitica and between Sereny-positive and -negative strains. A temperature-programmed gas-liquid chromatograph, equipped with a high-resolution Carbowax 20M coated capillary column, separated the volatiles from pyrolyzed whole cells preparations and cell wall fractions. The resulting pyrolysis elution patterns (pyrograms) were divided into 313 30-s time interval areas. The time interval areas were normalized in relation to the entire pyrogram area and were evaluated by stepwise linear discriminant analysis. The results of the PGLC-statistical analyses showed good correlation in prediction of the HeLa cell invasivity test. The technique of PGLC coupled with statistical analyses is objective, in contrast to traditional methods of determining pathogenicity of Y. enterocolitica.
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- Bottone E. J. Atypical Yersinia enterocolitica: clinical and epidemiological parameters. J Clin Microbiol. 1978 Jun;7(6):562–567. doi: 10.1128/jcm.7.6.562-567.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bottone E. J., Chester B., Malowany M. S., Allerhand J. Unusual Yersinia enterocolitica isolates not associated with mesenteric lymphadenitis. Appl Microbiol. 1974 May;27(5):858–861. doi: 10.1128/am.27.5.858-861.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cone R. D., Lechowich R. V. Differentiation of Clostridium botulinum types A, B, and E by pyrolysis-gas-liquid chromatography. Appl Microbiol. 1970 Jan;19(1):138–145. doi: 10.1128/am.19.1.138-145.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Emswiler B. S., Kotula A. W. Differentiation of Salmonella Serotypes by pyrolysis-gas-liquid chromatography of cell fragments. Appl Environ Microbiol. 1978 Jan;35(1):97–104. doi: 10.1128/aem.35.1.97-104.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feeley J. C., Wells J. G., Tsai T. F., Puhr N. D. Detection of enterotoxigenic and invasive strains of Yersinia enterocolitica. Contrib Microbiol Immunol. 1979;5:329–334. [PubMed] [Google Scholar]
- Haddadin J. M., Stirland R. M., Preston N. W., Collard P. Identification of Vibrio cholerae by pyrolysis gas-liquid chromatography. Appl Microbiol. 1973 Jan;25(1):40–43. doi: 10.1128/am.25.1.40-43.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lee W. H., McGrath P. P., Carter P. H., Eide E. L. The ability of some Yersinia enterocolitica strains to invade HeLa cells. Can J Microbiol. 1977 Dec;23(12):1714–1722. doi: 10.1139/m77-247. [DOI] [PubMed] [Google Scholar]
- Mehlman I. J., Aulisio C. C., Sanders A. C. Microbiological methods. Problems in the recovery and identification of Yersinia from food. J Assoc Off Anal Chem. 1978 Jul;61(4):761–771. [PubMed] [Google Scholar]
- Pai C. H., Mors V. Production of enterotoxin by Yersinia enterocolitica. Infect Immun. 1978 Mar;19(3):908–911. doi: 10.1128/iai.19.3.908-911.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reiner E., Hicks J. J., Ball M. M., Martin W. J. Rapid characterization of salmonella organisms by means of pyrolysis-gas-liquid chromatography. Anal Chem. 1972 May;44(6):1058–1061. doi: 10.1021/ac60314a031. [DOI] [PubMed] [Google Scholar]
- Reiner E. Identification of bacterial strains by pyrolysis-gas-liquid chromatography. Nature. 1965 Jun 19;206(990):1272–1273. doi: 10.1038/2061272b0. [DOI] [PubMed] [Google Scholar]
- Stern N. J., Kotula A. W., Pierson M. D. Differentiation of selected Enterobacteriaceae by pyrolysis-gas-liquid chromatography. Appl Environ Microbiol. 1979 Dec;38(6):1098–1102. doi: 10.1128/aem.38.6.1098-1102.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Une T. Studies on the pathogenicity of Yersinia enterocolitica. I. Experimental infection in rabbits. Microbiol Immunol. 1977;21(7):341–363. [PubMed] [Google Scholar]