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. 1980 Oct;40(4):809–820. doi: 10.1128/aem.40.4.809-820.1980

Attack on Lignified Grass Cell Walls by a Facultatively Anaerobic Bacterium

Danny E Akin 1
PMCID: PMC291665  PMID: 16345651

Abstract

A filamentous, facultatively anaerobic microorganism that attacked lignified tissue in forage grasses was isolated from rumen fluid with a Bermuda grass-containing anaerobic medium in roll tubes. The microbe, designated 7-1, demonstrated various colony and cellular morphologies under different growth conditions. Scanning electron microscopy revealed that 7-1 attacked lignified cell walls in aerobic and anaerobic culture. 7-1 predominately degraded tissues reacting positively for lignin with the chlorine-sulfite stain (i.e., sclerenchyma in leaf blades and parenchyma in stems) rather than the more resistant acid phloroglucinol-positive tissues (i.e., lignified vascular tissue and sclerenchyma ring in stems), although the latter tissues were occasionally attacked. Turbidimetric tests showed that 7-1 in anaerobic culture grew optimally at 39°C at a pH of 7.4 to 8.0. Tests for growth on plant cell wall carbohydrates showed that 7-1 grew on xylan and pectin slowly in aerobic cultures but not with pectin and only slightly with xylan in anaerobic culture. 7-1 was noncellulolytic as shown by filter paper tests. The microbe used the phenolic acids sinapic, ferulic, and p-coumaric acids as substrates for growth; the more highly methoxylated acids were used more effectively.

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Selected References

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  1. Akin D. E., Burdick D., Michaels G. E. Rumen bacterial interrelationships with plant tissue during degradation revealed by transmission electron microscopy. Appl Microbiol. 1974 Jun;27(6):1149–1156. doi: 10.1128/am.27.6.1149-1156.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Akin D. E. Ultrastructure of rigid and lignified forage tissue degradation by a filamentous rumen microorganism. J Bacteriol. 1976 Mar;125(3):1156–1162. doi: 10.1128/jb.125.3.1156-1162.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bellamy W. D. Biotechnology report: single cell proteins from cellulosic wastes. Biotechnol Bioeng. 1974 Jul;16(7):869–880. doi: 10.1002/bit.260160702. [DOI] [PubMed] [Google Scholar]
  4. Caldwell D. R., Bryant M. P. Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria. Appl Microbiol. 1966 Sep;14(5):794–801. doi: 10.1128/am.14.5.794-801.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Crawford D. L., Crawford R. L. Microbial degradation of lignocellulose: the lignin component. Appl Environ Microbiol. 1976 May;31(5):714–717. doi: 10.1128/aem.31.5.714-717.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Crawford D. L. Growth of Thermomonospora fusca on lignocellulosic pulps of varying lignin content. Can J Microbiol. 1974 Jul;20(7):1069–1072. doi: 10.1139/m74-167. [DOI] [PubMed] [Google Scholar]
  7. Gaillard B. D., Richards G. N. Presence of soluble lignin-carbohydrate complexes in the bovine rumen. Carbohydr Res. 1975 Jun;42(1):135–145. doi: 10.1016/s0008-6215(00)84106-3. [DOI] [PubMed] [Google Scholar]
  8. Stafford H. A. Histochemical & Biochemical Differences Between Lignin-Like Materials in Phleum pratense L. Plant Physiol. 1962 Sep;37(5):643–649. doi: 10.1104/pp.37.5.643. [DOI] [PMC free article] [PubMed] [Google Scholar]

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