Abstract
The influence of the inoculum size on growth and aflatoxin production was examined in Aspergillus parasiticus (NRRL 3145) by using a synthetic medium. The reduction in the number of spores by 4 to 5 log cycles either by serial dilution or by gamma irradiation caused a two fold increase in the toxin production. The decrease in the inoculum size induced a lag in growth of the culture, though the final yield of the mycelium over the 28-day experimental period was the same. The maximal accumulation of aflatoxin was observed on day 14 of incubation. A transition from the biphasic to monophasic pattern in aflatoxin production could be correlated with the size of the inoculum. The enhanced toxin production from dilute inocula was similar to that obtained with the surviving fraction of the spores after gamma irradiation (0 to 150 krads).
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Applegate K. L., Chipley J. R. Daily variations in the production of aflatoxins by Aspergillus flavus NRRL-3145 following exposure to 60 Co irradiation. J Appl Bacteriol. 1974 Sep;37(3):359–372. doi: 10.1111/j.1365-2672.1974.tb00452.x. [DOI] [PubMed] [Google Scholar]
- Arseculeratne S. N., Bandunatha C. H. Variation of aflatoxin content of cultures of aspergillus flavus with duration of incubation and its relation to studies on aflatoxin production. J Appl Bacteriol. 1972 Mar;35(1):43–52. doi: 10.1111/j.1365-2672.1972.tb03672.x. [DOI] [PubMed] [Google Scholar]
- Ciegler A., Peterson R. E., Lagoda A. A., Hall H. H. Aflatoxin production and degradation by Aspergillus flavus in 20-liter fermentors. Appl Microbiol. 1966 Sep;14(5):826–833. doi: 10.1128/am.14.5.826-833.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gottlieb D. Limited growth in fungi. Mycologia. 1971 May-Jun;63(3):619–629. [PubMed] [Google Scholar]
- JINKS J. L. Selection for adaptability to new environments in Aspergillus glaucus. J Gen Microbiol. 1959 Apr;20(2):223–236. doi: 10.1099/00221287-20-2-223. [DOI] [PubMed] [Google Scholar]
- Maggon K. K., Gupta S. K., Venkitasubramanian T. A. Biosynthesis of aflatoxins. Bacteriol Rev. 1977 Dec;41(4):822–855. doi: 10.1128/br.41.4.822-855.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shih C. H., Marth E. H. Some cultural conditions that control biosynthesis of lipid and aflatoxin by Aspergillus parasiticus. Appl Microbiol. 1974 Mar;27(3):452–456. doi: 10.1128/am.27.3.452-456.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Velasco J. Fluorometric measurement of aflatoxin adsorbed on florisil in minicolumns. J Assoc Off Anal Chem. 1975 Jul;58(4):757–763. [PubMed] [Google Scholar]