Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1980 Dec;40(6):1080–1085. doi: 10.1128/aem.40.6.1080-1085.1980

Single radial immunodiffusion method for screening Staphylococcal isolates for enterotoxin.

R F Meyer, M J Palmieri
PMCID: PMC291724  PMID: 6779703

Abstract

A direct system for screening large numbers of staphylococcal isolates for enterotoxin production has been developed. The system employs polyvalent (serotypes A, B, C, D, and E) immunodiffusion assay slides in conjunction with a multiple-culturing system for toxin production. With the combined system, as many as 50 cultures can be screened simultaneously on a single assay slide having a sensitivity of about 0.3 microgram/ml. The system should be useful for detecting potential enterotoxin in foods containing a predominance of non-enterotoxigenic strains.

Full text

PDF
1080

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CASMAN E. P., BENNETT R. W. CULTURE MEDIUM FOR THE PRODUCTION OF STAPHYLOCOCCAL ENTEROTOXIN A. J Bacteriol. 1963 Jul;86:18–23. doi: 10.1128/jb.86.1.18-23.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Casman E. P., Bennett R. W., Dorsey A. E., Stone J. E. The micro-slide gel double diffusion test for the detection and assay of staphylococcal enterotoxins. Health Lab Sci. 1969 Oct;6(4):185–198. [PubMed] [Google Scholar]
  3. Donnelly C. B., Leslie J. E., Black L. A., Lewis K. H. Serological identification of enterotoxigenic staphylococci from cheese. Appl Microbiol. 1967 Nov;15(6):1382–1387. doi: 10.1128/am.15.6.1382-1387.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lumpkins E. D., Sr Method for staining and preserving agar gel diffusion plates. Appl Microbiol. 1972 Sep;24(3):499–500. doi: 10.1128/am.24.3.499-500.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  6. Noleto A. L., Bergdoll M. S. Staphylococcal enterotoxin production in the presence of non-enterotoxigenic staphylococci. Appl Environ Microbiol. 1980 Jun;39(6):1167–1171. doi: 10.1128/aem.39.6.1167-1171.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Robbins R., Gould S., Bergdoll M. Detecting the enterotoxigenicity of Staphylococcus aureus strains. Appl Microbiol. 1974 Dec;28(6):946–950. doi: 10.1128/am.28.6.946-950.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES