Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1985 Oct;50(4):1064–1067. doi: 10.1128/aem.50.4.1064-1067.1985

Improvement of a Wine Saccharomyces cerevisiae Strain by a Breeding Program

Patrizia Romano 1, M Grazia Soli 1, Giovanna Suzzi 1, Luigi Grazia 1, Carlo Zambonelli 1,*
PMCID: PMC291794  PMID: 16346903

Abstract

Hybridization by spore conjugation was used to develop new and improved wine yeasts of Saccharomyces cerevisiae. The procedure was achieved with diploid, homothallic strains with high sporulation frequency and high spore viability. The method was verified by crossing flocculent and non-H2S-forming strains. Single-spore descendants of the hybrids were studied by tetrad analysis with regard to the aforementioned characters and the other two winemaking traits, i.e., ethanol production and fermentation rate. A highly flocculent, non-H2S-forming wine yeast strain with a high fermentation rate and high ethanol production was obtained.

Full text

PDF
1064

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BUCHER T., REDETZKI H. Eine spezifische photometrische Bestimmung von Athylalkohol auf fermentativen Wege. Klin Wochenschr. 1951 Sep 15;29(35-36):615–616. doi: 10.1007/BF01485653. [DOI] [PubMed] [Google Scholar]
  2. CASTELLI T. Les agents de la fermentation vinaire. Arch Mikrobiol. 1954;20(4):323–342. [PubMed] [Google Scholar]
  3. Lindegren C. C., Lindegren G. A New Method for Hybridizing Yeast. Proc Natl Acad Sci U S A. 1943 Oct 15;29(10):306–308. doi: 10.1073/pnas.29.10.306. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. NICKERSON W. J. Reduction of inorganic substances by yeasts. I. Extracellular reduction of sulfite by species of Candida. J Infect Dis. 1953 Jul-Aug;93(1):43–56. doi: 10.1093/infdis/93.1.43. [DOI] [PubMed] [Google Scholar]
  5. THORNE R. S. W. The genetics of flocculence in Saccharomyces cerevisiae. Cr Trav Lab Carlsberg Ser Physiol. 1951;25(4):101–139. [PubMed] [Google Scholar]
  6. Takano I., Oshima Y. Allelism tests among various homothallism-controlling genes and gene systems in Saccharomyces. Genetics. 1970 Feb;64(2):229–238. doi: 10.1093/genetics/64.2.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Thornton R. J., Eschenbruch R. Homothallism in wine yeasts. Antonie Van Leeuwenhoek. 1976;42(4):503–509. doi: 10.1007/BF00410181. [DOI] [PubMed] [Google Scholar]
  8. Wainwright T. Hydrogen sulphide production by yeast under conditions of methionine, pantothenate or vitamin B6 deficiency. J Gen Microbiol. 1970 Apr;61(1):107–119. doi: 10.1099/00221287-61-1-107. [DOI] [PubMed] [Google Scholar]
  9. Zambonelli C., Mutinelli P. Biosynthesis of sulphur amoni acids in Saccharomyces cerevisiae. I. Genetic analysis of leaky mutants of sulphite reductase. Arch Microbiol. 1975 Mar 10;102(3):247–251. doi: 10.1007/BF00428375. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES