Abstract
A selective and differential growth medium was developed for detection of Clostridium botulinum types A, B, and F. The medium consisted of peptone-glucose-yeast extract agar supplemented with cycloserine, 250 micrograms/ml; sulfamethoxazole, 76 micrograms/ml; and trimethoprim, 4 micrograms/ml as selective inhibitors and various types and levels of botulinal antibodies for type differentiation in the immunodiffusion reaction. Growth of proteolytic types of C. botulinum were not affected by the incorporation of the selective agents, but some nonproteolytic types were suppressed. Cross-reactions between types A and B were visually distinguishable, whereas cross-reactions between type F and Clostridium sporogenes did not occur at the optimum antibody titer. Optimum antibody titer varied with toxin type. The proposed selective differential medium should be valuable in isolating and typing of proteolytic C. botulinum types A, B, and F from samples containing mixed microbial populations.
Full text
PDF

Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dezfulian M., McCroskey L. M., Hatheway C. L., Dowell V. R., Jr Selective medium for isolation of Clostridium botulinum from human feces. J Clin Microbiol. 1981 Mar;13(3):526–531. doi: 10.1128/jcm.13.3.526-531.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ferreira J. L., Hamdy M. K., Zapatka F. A., Hebert W. O. Immunodiffusion method for detection of type A Clostridium botulinum. Appl Environ Microbiol. 1981 Dec;42(6):1057–1061. doi: 10.1128/aem.42.6.1057-1061.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LAMANNA C., LOWENTHAL J. P. The lack of identity between hemagglutinin and the toxin of type A botulinal organism. J Bacteriol. 1951 Jun;61(6):751–752. doi: 10.1128/jb.61.6.751-752.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Swenson J. M., Thornsberry C., McCroskey L. M., Hatheway C. L., Dowell V. R., Jr Susceptibility of Clostridium botulinum to thirteen antimicrobial agents. Antimicrob Agents Chemother. 1980 Jul;18(1):13–19. doi: 10.1128/aac.18.1.13. [DOI] [PMC free article] [PubMed] [Google Scholar]