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. Author manuscript; available in PMC: 2010 Aug 9.
Published in final edited form as: Oncogene. 2008 Sep 22;28(1):41–51. doi: 10.1038/onc.2008.359

Figure 2.

Figure 2

Redistribution of cleavage and polyadenylation-specific factor 3 (CPSF3) upon induction of cellular stress response 1 (CSR1) expression. (a) Immunofluorescence staining of PWC1 cells. PWC1 cells (PC3 harboring pCDNA4-CSR1/pCDNA6) treated with or without tetracycline were stained with antibodies specific for CSR1 or CPSF3. The images were obtained after incubation with rhodamine-conjugated anti-rabbit (CSR1) and fluorescein-conjugated anti-goat secondary antibodies. (b) Immunoblot analysis of CPSF3 distribution. The upper panel: CSR1 expression in PWC1, PWC3, PWR1 and PWR2 cells. The lower panel: nuclear and cytoplasmic proteins from PC3 harboring wild-type (PWC1 and PWC3) or mutant (PWR1 and PWR2) were separated by electrophoresis on an 8% SDS–polyacrylamide gel electrophoresis (PAGE) and immunoblotted with antibodies specific for CPSF3, GAPDH or histone 3. (c) Immunoblot analysis of CPSF3 distribution in DU145 cells. The upper panel: CSR1 expression in DWC1 (DU145 harboring pCDNA4-CSR1/pCDNA6, lanes 1 and 3), DWC5 (lanes 2 and 4) or mutant cells (DWR3 (lanes 5 and 7) and DWR4 (lanes 6 and 8)). The lower panel: nuclear and cytoplasmic proteins from DU145 cells expressing wild-type CSR1 (DWC1 (lanes 1, 3, 9 and 11) and DWC5 (lanes 2, 4, 10 and 12)) or mutant CSR1 (DWR3 (lanes 5, 7, 13 and 15) and DWR4 (lanes 6, 8, 14 and 16)) were separated by electrophoresis on an 8% SDS–PAGE and immunoblotted with antibodies specific for CPSF3, GAPDH or histone 3. (d) Immunoblot analysis of CPSF3 distribution in LNCaP cells. The upper panel: CSR1 expression in LWC6 (LNCaP harboring pCDNA4-CSR1/pCDNA6, lanes 1 and 3) and LWC7 (lanes 2 and 4) or mutants (LWR2 (lanes 5 and 7) and LWR4 (lanes 6 and 8)) cells. The lower panel: nuclear and cytoplasmic proteins from LNCaP harboring wild-type (LWC6 (lanes 1, 3, 9 and 11) and LWC7 (lanes 2, 4, 10 and 12)) or mutant (LWR2 (lanes 5, 7, 13 and 15) and LWR4 (lanes 6, 8, 14 and 16)) CSR1 were separated by electrophoresis on an 8% SDS–PAGE and immunoblotted with antibodies specific for CPSF3, GAPDH or histone 3. U: uninduced;I: induced with tetracycline.