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. Author manuscript; available in PMC: 2010 Aug 9.
Published in final edited form as: Oncogene. 2008 Sep 22;28(1):41–51. doi: 10.1038/onc.2008.359

Figure 5.

Figure 5

Interaction with cleavage and polyadenylation-specific factor 3 (CPSF3) is required for cellular stress response 1 (CSR1)-mediated cell death. (a) Immunoblot analysis of CSR1, CPSF3 and mutant CSR1 expression (ΔCSR1) expression. PWC1 cells (lanes 1 through 6) were transfected with scramble small interfering RNA (siRNA) (sScr, lanes 2 and 5) or siRNA specific for CSR1 (sCSR1, lane 3) or siRNA specific for CPSF3 (sCPSF3, lane 6), and treated with tetracycline (lanes 1–3) or not (lanes 4–6), or PWR1 (PC3 harboring mutant CSR1) cells were treated with tetracycline (lane 8) or not (lane 7). (b) Fluorescence-activated cell sorting analysis of cell death in cells used in (a). These cells were stained with Alexa Fluoro 488-labeled annexin V and propidium iodide (PI). The cells were sorted and quantified in a LSR-II flow cytometer as described in the Materials and methods section.