Table 1.
Cell death induced by CSR1
| CSR1 induction | PI staining alone |
Annexin V alone |
Annexin V+PI |
|||
|---|---|---|---|---|---|---|
| −CSR1 (%) | +CSR1 (%) | −CSR1 (%) | +CSR1 (%) | −CSR1 (%) | +CSR1 (%) | |
| PWC1 | 0.3 | 7.6 | 1.1 | 22.2 | 2.5 | 20.9 |
| PWC3 | 0.5 | 7.5 | 1.3 | 19.8 | 1.3 | 18.7 |
| PDC1 | 1.0 | 8.5 | 0.9 | 20.8 | 1.0 | 19.9 |
| PDC2 | 1.2 | 7.9 | 0.7 | 21.6 | 0.5 | 18.5 |
| DWC1 | 5.7 | 3.8 | 0 | 7.3 | 0.1 | 16.0 |
| DWC5 | 6.9 | 4.4 | 1.3 | 8.3 | 0.6 | 15.7 |
| LWC6 | 10 | 6 | 0 | 3.6 | 0.4 | 16.3 |
| LWC7 | 9.8 | 6.8 | 0.3 | 3.5 | 0.9 | 15.9 |
| PWC1+scramble siRNA | 0.7 | 4.8 | 0.6 | 15.2 | 1.9 | 16.8 |
| PWC1+CSR1 siRNA | 0.5 | 0.7 | 3.0 | 2.0 | 3.7 | 3.5 |
| PWC1+CPSF3 siRNA | 3.5 | 3.4 | 21.7 | 20.1 | 20.2 | 19.2 |
| PDC1+scramble siRNA | 1.2 | 8.2 | 0.5 | 20.1 | 1.4 | 19.3 |
| PDC1+CSR1 siRNA | 1.3 | 2.2 | 1.0 | 2.9 | 1.5 | 3.1 |
| PDC1+CPSF3 siRNA | 9.5 | 9.9 | 16.1 | 16.6 | 16.0 | 16.8 |
| PWR1 | 2.8 | 3.1 | 0.8 | 0.9 | 1.0 | 1.3 |
| PWR2 | 2.5 | 2.8 | 0.7 | 0.5 | 1.1 | 0.8 |
| DWR3 | 8.9 | 7.9 | 2.9 | 1.1 | 1.4 | 1.1 |
| DWR4 | 7.3 | 10.6 | 2.3 | 1.7 | 2.4 | 4.4 |
| LWR2 | 10.7 | 11.3 | 0.8 | 0.9 | 0.7 | 1.4 |
| LWR4 | 12.8 | 10.5 | 1.1 | 1.4 | 0.6 | 0.9 |
Abbreviations: CPSF3, cleavage and polyadenylation-specific factor 3; CSR1, cellular stress response 1; PI, propidium iodide; siRNA, small interfering RNA.
PWC1, PWC3, PDC1 and PDC2 are four different isolates of PC3 cells harboring pCDNA4-CSR1/pCDNA6 constructs.