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. Author manuscript; available in PMC: 2010 Aug 10.
Published in final edited form as: Chromatographia. 2010 Jan 1;71(1-2):37–53. doi: 10.1365/s10337-009-1409-0

Fig. 13.

Fig. 13

The dependence of fluorescence intensity (excitation and emission wavelengths of 360 and 480 nm, respectively) on the concentration of 3-NT after 3-NT reduction and tagging of whole phosphorylase b (open squares) or protein digest (solid squares) with 2 mM ABS and 20 μM K3Fe(CN)6. The total amount of protein in the samples was maintained at 100 μg; the 3-NT content was altered through mixing of native and nitrated protein at certain ratios