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. 1971 Jan;50(1):49–59. doi: 10.1172/JCI106483

Resting transmembrane potential difference of skeletal muscle in normal subjects and severely ill patients

J N Cunningham Jr 1, N W Carter 1, F C Rector Jr 1, D W Seldin 1
PMCID: PMC291892  PMID: 5101298

Abstract

The resting membrane potential difference (Em) of skeletal muscle was measured in 26 normal human subjects, 7 patients with mild illness, and 21 patients with severe, debilitating medical disorders. A closed transcutaneous approach to the muscle was made by needle puncture and the Em was measured utilizing standard Ling electrodes. Measurements revealed an Em of -88 ±3.8 mv in healthy subjects and -89 ±2.1 mv in patients hospitalized for minor medical problems. The mean Em in 21 in-hospital patients, judged to be severely ill clinically from a variety of causes, was -66.3 ±9.0 mv. Open deltoid muscle biopsies were performed in 7 of the healthy subjects and in 13 of the severely ill group. Estimation of the intra-extracellular water partition was made by calculating the chloride space from the previously measured Em. Analysis of the muscle samples revealed no significant difference in the intra-extracellular potassium ratios of the two groups biopsied. Intracellular Na+ concentrations were uniformly increased in the muscle samples of the severely ill subjects and averaged 42.3% higher than those of the normal subjects. The mechanisms which might account for the elevation of intracellular Na+ and a depression of Em independent of changes in intra-extracellular K+ ratios are discussed and it is suggested that this defect may be a generalized cellular abnormality which is a common quality of serious illnesses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERANEK R. INTRACELLULAR STIMULATION MYOGRAPHY IN MAN. Electroencephalogr Clin Neurophysiol. 1964 Mar;16:301–304. doi: 10.1016/0013-4694(64)90114-2. [DOI] [PubMed] [Google Scholar]
  2. BOLTE H. D., RIECKER G., ROHL D. [Measurements of the membrane potential of single cross-striated muscles of man in situ. Normal values]. Klin Wochenschr. 1963 Apr 15;41:356–359. doi: 10.1007/BF01487862. [DOI] [PubMed] [Google Scholar]
  3. Brooks J. E., Hongdalarom T. Intracellular electromyography. Resting and action potentials in normal human muscle. Arch Neurol. 1968 Mar;18(3):291–300. doi: 10.1001/archneur.1968.00470330081008. [DOI] [PubMed] [Google Scholar]
  4. CONWAY E. J. Nature and significance of concentration relations of potassium and sodium ions in skeletal muscle. Physiol Rev. 1957 Jan;37(1):84–132. doi: 10.1152/physrev.1957.37.1.84. [DOI] [PubMed] [Google Scholar]
  5. CREUTZFELDT O. D., ABBOTT B. C., FOWLER W. M., PEARSON C. M. MUSCLE MEMBRANE POTENTIALS IN EPISODIC ADYNAMIA. Electroencephalogr Clin Neurophysiol. 1963 Jun;15:508–519. doi: 10.1016/0013-4694(63)90071-3. [DOI] [PubMed] [Google Scholar]
  6. Carter N. W., Rector F. C., Jr, Campion D. S., Seldin D. W. Measurement of intracellular pH of skeletal muscle with pH-sensitive glass microelectrodes. J Clin Invest. 1967 Jun;46(6):920–933. doi: 10.1172/JCI105598. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Darrow D. C., Schwartz R., Iannucci J. F., Coville F. THE RELATION OF SERUM BICARBONATE CONCENTRATION TO MUSCLE COMPOSITION. J Clin Invest. 1948 Mar;27(2):198–208. doi: 10.1172/JCI101934. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eberstein A., Goodgold J. Slow and fast twitch fibers in human skeletal muscle. Am J Physiol. 1968 Sep;215(3):535–541. doi: 10.1152/ajplegacy.1968.215.3.535. [DOI] [PubMed] [Google Scholar]
  9. FRANK K., FUORTES M. G. Potentials recorded from the spinal cord with microelectrodes. J Physiol. 1955 Dec 29;130(3):625–654. doi: 10.1113/jphysiol.1955.sp005432. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Goodgold J., Eberstein A. Transmembrane potentials of human muscle cells in vivo. Exp Neurol. 1966 Jul;15(3):338–346. doi: 10.1016/0014-4886(66)90056-2. [DOI] [PubMed] [Google Scholar]
  11. HODGKIN A. L., KATZ B. The effect of sodium ions on the electrical activity of giant axon of the squid. J Physiol. 1949 Mar 1;108(1):37–77. doi: 10.1113/jphysiol.1949.sp004310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McComas A. J., Mrozek K., Gardner-Medwin D., Stanton W. H. Electrical properties of muscle fibre membranes in man. J Neurol Neurosurg Psychiatry. 1968 Oct;31(5):434–440. doi: 10.1136/jnnp.31.5.434. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. NORRIS F. H., Jr Unstable membrane potential in human myotonic muscle. Electroencephalogr Clin Neurophysiol. 1962 Apr;14:197–201. doi: 10.1016/0013-4694(62)90029-9. [DOI] [PubMed] [Google Scholar]
  14. PRICE H. M., HOWES E. L., Jr, SHELDON D. B., HUTSON O. D., FITZGERALD R. T., BLUMBERG J. M., PEARSON C. M. AN IMPROVED BIOPSY TECHNIQUE FOR LIGHT AND ELECTRON MICROSCOPIC STUDIES OF HUMAN SKELETAL MUSCLE. Lab Invest. 1965 Feb;14:194–199. [PubMed] [Google Scholar]
  15. WELT L. G., SACHS J. R., MCMANUS T. J. AN ION TRANSPORT DEFECT IN ERYTHROCYTES FROM UREMIC PATIENTS. Trans Assoc Am Physicians. 1964;77:169–181. [PubMed] [Google Scholar]
  16. Welt L. G. Membrane transport defect: the sick cell. Trans Assoc Am Physicians. 1967;80:217–226. [PubMed] [Google Scholar]

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