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. 1971 Jun;50(6):1272–1275. doi: 10.1172/JCI106605

Marrow grafts between canine siblings matched by serotyping and mixed leukocyte culture

Rainer Storb 1,2, Robert H Rudolph 1,2, E Donnall Thomas 1,2
PMCID: PMC292057  PMID: 4931083

Abstract

Marrow grafts were carried out between 16 canine sibling donor-recipient pairs. The pairs were matched by serological histocompatibility testing and were nonreactive in a one-way mixed leukocyte culture. Recipients were prepared for transplantation by 1500-1580 R of total body irradiation. Donor marrow was infused within 4 hr of irradiation. Recipients were not given immunosuppressive drug therapy after grafting. All 16 recipients showed evidence of prompt and sustained allogeneic marrow engraftment. Six died between 30 and 128 days after grafting with graft-versus-host disease, and three died between days 72 and 230 with pneumonia but no evidence of graft-versus-host disease with the exception of lymphoid atrophy. Seven recipients survived without graft-versus-host disease and are in excellent health between 200 and 684 after grafting.

In summary, fatal graft-versus-host disease was observed in a number of canine recipients despite matching with their sibling donor by serological histocompatibility testing and by mixed leukocyte culture in a manner similar to that employed to define human HL-A matched sibling pairs. The graft-versus-host disease in these matched siblings developed more slowly than that observed in mismatched dogs, but the ultimate death of approximately half of the matched recipients emphasizes the need for posttransplantation immunosuppression even in this “compatible” situation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amos D. B., Bach F. H. Phenotypic expressions of the major histocompatibility locus in man (HL-A): leukocyte antigens and mixed leukocyte culture reactivity. J Exp Med. 1968 Oct 1;128(4):623–637. doi: 10.1084/jem.128.4.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CONGDON C. C., URSO I. S. Homologous bone marrow in the treatment of radiation injury in mice. Am J Pathol. 1957 Jul-Aug;33(4):749–767. [PMC free article] [PubMed] [Google Scholar]
  3. Dausset J., Colombani J., Legrand L., Feingold N., Rapaport F. T. Genetic and biological aspects of the HL-A system of human histocompatibility. Blood. 1970 May;35(5):591–612. [PubMed] [Google Scholar]
  4. Epstein R. B., Storb R., Clift R. A., Thomas E. D. Transplantation of stored allogeneic bone marrow in dogs selected by histocompatibility typing. Transplantation. 1969 Oct;8(4):496–501. doi: 10.1097/00007890-196910000-00022. [DOI] [PubMed] [Google Scholar]
  5. Epstein R. B., Storb R., Ragde H., Thomas E. D. Cytotoxic typing antisera for marrow grafting in littermate dogs. Transplantation. 1968 Jan;6(1):45–58. doi: 10.1097/00007890-196801000-00005. [DOI] [PubMed] [Google Scholar]
  6. GORER P. A., BOYSE E. A. Pathological changes in F1 hybrid mice following transplantation of spleen cells from donors of the parental strains. Immunology. 1959 Apr;2(2):182–193. [PMC free article] [PubMed] [Google Scholar]
  7. Mollen N., Cannon F. D., Ferrebee J. W., St John D. Lymphocyte typing in allografted beagles. Transplantation. 1968 Nov;6(8):939–940. doi: 10.1097/00007890-196811000-00009. [DOI] [PubMed] [Google Scholar]
  8. PORTER K. A. Graft-versus-host reactions in the rabbit. Br J Cancer. 1960 Mar;14:66–76. doi: 10.1038/bjc.1960.8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rudolph R. H., Hered B., Epstein R. B., Thomas E. D. Canine mixed leukocyte reactivity and transplantation antigens. Transplantation. 1969 Aug;8(2):141–146. doi: 10.1097/00007890-196908000-00006. [DOI] [PubMed] [Google Scholar]
  10. SWISHER S. N., YOUNG L. E. The blood grouping systems of dogs. Physiol Rev. 1961 Jul;41:495–520. doi: 10.1152/physrev.1961.41.3.495. [DOI] [PubMed] [Google Scholar]
  11. Storb R., Epstein R. B., Bryant J., Ragde H., Thomas E. D. Marrow grafts by combined marrow and leukocyte infusions in unrelated dogs selected by histocompatibility typing. Transplantation. 1968 Jul;6(4):587–593. doi: 10.1097/00007890-196807000-00011. [DOI] [PubMed] [Google Scholar]
  12. Storb R., Epstein R. B., Graham T. C., Thomas E. D. Methotrexate regimens for control of graft-versus-host disease in dogs with allogeneic marrow grafts. Transplantation. 1970 Mar;9(3):240–246. doi: 10.1097/00007890-197003000-00007. [DOI] [PubMed] [Google Scholar]
  13. Storb R., Graham T. C., Shiurba R., Thomas E. D. Treatment of canine graft-versus-host disease with methotrexate and cyclo-phosphamide following bone marrow transplantation from histoincompatible donors. Transplantation. 1970 Aug;10(2):165–172. doi: 10.1097/00007890-197008000-00003. [DOI] [PubMed] [Google Scholar]
  14. THOMAS E. D., ASHLEY C. A., LOCHTE H. L., Jr, JARETZKI A., 3rd, SAHLER O. D., FERREBEE J. W. Homografts of bone marrow in dogs after lethal total-body radiation. Blood. 1959 Jun;14(6):720–736. [PubMed] [Google Scholar]
  15. Thomas E. D., LeBlond R., Graham T., Storb R. Marrow infusions in dogs given midlethal or lethal irradiation. Radiat Res. 1970 Jan;41(1):113–124. [PubMed] [Google Scholar]
  16. de VRIES M., CROUCH B. G., van PUTTEN L., van BEKKUM D. Pathologic changes in irradiated monkeys treated with bone marrow. J Natl Cancer Inst. 1961 Jul;27:67–97. [PubMed] [Google Scholar]

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