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. 1971 Jul;50(7):1379–1385. doi: 10.1172/JCI106620

Accelerated reabsorption in the proximal tubule produced by volume depletion

Michael W Weiner 1,2,3, Edward J Weinman 1,2,3, Michael Kashgarian 1,2,3, John P Hayslett 1,2,3
PMCID: PMC292075  PMID: 5090054

Abstract

The renal response to chronic depletion of extracellular volume was examined using the techniques of micropuncture. Depletion of salt and water was produced by administration of furosemide to rats maintained on a sodium-free diet. There was a marked fall in body weight, plasma volume, and glomerular filtration rate. The intrinsic reabsorptive capacity of the proximal tubule, measured by the split-droplet technique, was greatly enhanced. The acceleration of proximal fluid reabsorption could not be accounted for by changes in filtration rate, tubular geometry, or aldosterone secretion. The half-time of droplet reabsorption in the distal tubule was not altered by sodium depletion.

An increase in the reabsorption of fluid in the proximal tubule, as demonstrated directly in the present experiments, provides an explanation for a variety of clinical phenomena associated with volume depletion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BELL N. H., SCHEDL H. P., BARTTER F. C. AN EXPLANATION FOR ABNORMAL WATER RETENTION AND HYPOOSMOLALITY IN CONGESTIVE HEART FAILURE. Am J Med. 1964 Mar;36:351–360. doi: 10.1016/0002-9343(64)90161-5. [DOI] [PubMed] [Google Scholar]
  2. Bank N., Koch K. M., Aynedjian H. S., Aras M. Effect of changes in renal perfusion pressure on the suppression of proximal tubular sodium reabsorption due to saline loading. J Clin Invest. 1969 Feb;48(2):271–283. doi: 10.1172/JCI105983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brenner B. M., Berliner R. W. Relationship between extracellular volume and fluid reabsorption by the rat nephron. Am J Physiol. 1969 Jul;217(1):6–12. doi: 10.1152/ajplegacy.1969.217.1.6. [DOI] [PubMed] [Google Scholar]
  4. Brenner B. M., Falchuk K. H., Keimowitz R. I., Berliner R. W. The relationship between peritubular capillary protein concentration and fluid reabsorption by the renal proximal tubule. J Clin Invest. 1969 Aug;48(8):1519–1531. doi: 10.1172/JCI106118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dirks J. H., Cirksena W. J., Berliner R. W. Micropuncture study of the effect of various diuretics on sodium reabsorption by the proximal tubules of the dog. J Clin Invest. 1966 Dec;45(12):1875–1885. doi: 10.1172/JCI105492. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Earley L. E., Orloff J. THE MECHANISM OF ANTIDIURESIS ASSOCIATED WITH THE ADMINISTRATION OF HYDROCHLOROTHIAZIDE TO PATIENTS WITH VASOPRESSIN-RESISTANT DIABETES INSIPIDUS. J Clin Invest. 1962 Nov;41(11):1988–1997. doi: 10.1172/JCI104657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GERTZ K. H. [Transtubular sodium chloride transport and permeability for nonelectrolytes in the proximal and distal convolution of the rat kidney]. Pflugers Arch Gesamte Physiol Menschen Tiere. 1963;276:336–356. [PubMed] [Google Scholar]
  8. GOLDSMITH C., BEASLEY H. K., WHALLEY P. J., RECTOR F. C., Jr, SELDIN D. W. The effect of salt deprivation on the urinary concentrating mechanism in the dog. J Clin Invest. 1961 Nov;40:2043–2052. doi: 10.1172/JCI104430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gann D. S., Wright H. K. Increased renal sodium reabsorption after depletion of the extracellular or intravascular fluid volumes. J Surg Res. 1966 May;6(5):196–204. doi: 10.1016/s0022-4804(66)80016-1. [DOI] [PubMed] [Google Scholar]
  10. Gertz K. H., Mangos J. A., Braun G., Pagel H. D. On the glomerular tubular balance in the rat kidney. Pflugers Arch Gesamte Physiol Menschen Tiere. 1965 Sep 15;285(4):360–372. doi: 10.1007/BF00363236. [DOI] [PubMed] [Google Scholar]
  11. Hayslett J. P., Domoto D. T., Kashgarian M., Epstein F. H. Role of physical factors in the natriuresis induced by acetylcholine. Am J Physiol. 1970 Mar;218(3):880–885. doi: 10.1152/ajplegacy.1970.218.3.880. [DOI] [PubMed] [Google Scholar]
  12. Hayslett J. P., Kashgarian M., Epstein F. H. Changes in proximal and distal tubular reabsorption produced by rapid expansion of extracellular fluid. J Clin Invest. 1967 Jul;46(7):1254–1263. doi: 10.1172/JCI105618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hayslett J. P., Kashgarian M., Epstein F. H. Functional correlates of compensatory renal hypertrophy. J Clin Invest. 1968 Apr;47(4):774–799. doi: 10.1172/JCI105772. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Knox F. G., Howards S. S., Wright F. S., Davis B. B., Berliner R. W. Effect of dilution and expansion of blood volume on proximal sodium reabsorption. Am J Physiol. 1968 Nov;215(5):1041–1048. doi: 10.1152/ajplegacy.1968.215.5.1041. [DOI] [PubMed] [Google Scholar]
  15. Landwehr D. M., Klose R. M., Giebisch G. Renal tubular sodium and water reabsorption in the isotonic sodium chloride-loaded rat. Am J Physiol. 1967 Jun;212(6):1327–1333. doi: 10.1152/ajplegacy.1967.212.6.1327. [DOI] [PubMed] [Google Scholar]
  16. Landwehr D. M., Schnermann J., Klose R. M., Giebisch G. Effect of reduction in filtration rate on renal tubular sodium and water reabsorption. Am J Physiol. 1968 Sep;215(3):687–695. doi: 10.1152/ajplegacy.1968.215.3.687. [DOI] [PubMed] [Google Scholar]
  17. MARIEB N. J., MULROW P. J. ROLE OF THE RENIN-ANGIOTENSIN SYSTEM IN THE REGULATION OF ALDOSTERONE SECRETION IN THE RAT. Endocrinology. 1965 Apr;76:657–664. doi: 10.1210/endo-76-4-657. [DOI] [PubMed] [Google Scholar]
  18. Malnic G., Klose R. M., Giebisch G. Microperfusion study of distal tubular potassium and sodium transfer in rat kidney. Am J Physiol. 1966 Sep;211(3):548–559. doi: 10.1152/ajplegacy.1966.211.3.548. [DOI] [PubMed] [Google Scholar]
  19. McCance R. A., Widdowson E. M. The secretion of urine in man during experimental salt deficiency. J Physiol. 1937 Nov 26;91(2):222–231. doi: 10.1113/jphysiol.1937.sp003554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rector F. C., Jr, Sellman J. C., Martinez-Maldonado M., Seldin D. W. The mechanism of suppression of proximal tubular reabsorption by saline infusions. J Clin Invest. 1967 Jan;46(1):47–56. doi: 10.1172/JCI105510. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. SCHEDL H. P., BARTTER F. C. An explanation for and experimental correction of the abnormal water diuresis in cirrhosis. J Clin Invest. 1960 Feb;39:248–261. doi: 10.1172/JCI104035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. STEIN R. M., LEVITT B. H., GOLDSTEIN M. H., PORUSH J. G., EISNER G. M., LEVITT M. F. The effects of salt restriction on the renal concentrating operation in normal, hydropenic man. J Clin Invest. 1962 Dec;41:2101–2111. doi: 10.1172/JCI104668. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Spitzer A., Windhager E. E. Effect of peritubular oncotic pressure changes on proximal tubular fluid reabsorption. Am J Physiol. 1970 Apr;218(4):1188–1193. doi: 10.1152/ajplegacy.1970.218.4.1188. [DOI] [PubMed] [Google Scholar]
  24. Steele T. H., Oppenheimer S. Factors affecting urate excretion following diuretic administration in man. Am J Med. 1969 Oct;47(4):564–574. doi: 10.1016/0002-9343(69)90187-9. [DOI] [PubMed] [Google Scholar]
  25. WESSON L. G., Jr Glomerular and tubular factors in the renal excretion of sodium chloride. Medicine (Baltimore) 1957 Sep;36(3):281–396. doi: 10.1097/00005792-195709000-00002. [DOI] [PubMed] [Google Scholar]
  26. Wiederholt M., Stolte H., Brecht J. P., Hierholzer K. Mikropunktionsuntersuchungen über den Einfluss von Aldosteron, Cortison und Dexamethason auf die renale Natriumresorption adrenalektomierter Ratten. Pflugers Arch Gesamte Physiol Menschen Tiere. 1966;292(4):316–333. [PubMed] [Google Scholar]

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